γ-神经突触核蛋白基因沉默对子宫内膜癌HEC-1A细胞周期的影响  

Effects of SNCG gene silencing on the cell cycle of endometrial carcinoma HEC-1A cells

作　　者：陈少花 范余娟 李文怡[1] 徐红[1] 陈泓[1] 范江涛[1] 

机构地区：[1]广西医科大学第一附属医院妇科,广西南宁市530021 [2]广东省深圳市光明新区人民医院妇科,518106

出　　处：《实用医学杂志》2017年第22期3686-3689,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:81460396)

摘　　要：目的观察γ-神经突触核蛋白(SNCG)基因稳定沉默后人子宫内膜癌HEC-1A细胞生长周期的变化。方法采用流式细胞术检测SNCG基因沉默后HEC-1A细胞周期变化;通过激光扫描共聚焦显微镜观察吖啶橙染色后G2/M期百分比变化。结果流式细胞术结果显示,SNCG基因沉默后HEC-1A细胞处于G_1和G_2/M期增多,处于S期减少,差异有统计学意义(P<0.05);共聚焦显微镜观察结果显示,SNCG基因沉默后HEC-1A细胞处于G_2/M期百分比增高,差异有统计学意义(P<0.05)。结论两个方法共同验证了慢病毒质粒载体shRNA干扰后SNCG基因稳定沉默的HEC-1A细胞周期阻滞于G_2/M期,提示SNCG基因与子宫内膜癌细胞生长周期密切相关。Objective To observe changes of cell cycle after stable silencing of SNCG gene in human endometrial carcinoma HEC- 1A cells. Methods Flow cytometry was used to observe the changes of SNCG stable silencing HEC-1A cell growth cycle. The percentage of G2/M phase of HCE-1A cells after acridine orange staining was observed by laser scanning confocal microscopy. Results Flow cytometry results showed that the group of SNCG stable silencing in G1 and G2/M phase of the cells increased, but the percentage of cells in the S phase were decreased, the difference were both statistically significant （P 〈 0.05 ）. The results of cells after confocal microscopy observation of acridine orange staining, the group of SNCG stable silencing were observed in G2/M phase high percentage were statistically significant （P 〈 0.05）. Conclusion Two experimental methods commonly validated that the cell cycle of SNCG stable silencing on HEC- 1A cells was arrested in G2/M phase, suggesting that SNCG gene is closely related to the cell growth cycle of endometrial carcinoma.

关 键 词：子宫内膜癌 SNCG 激光扫描共聚焦显微镜 细胞周期 

分 类 号：R737.33[医药卫生—肿瘤]