非小细胞肺癌组织中snail基因的表达及意义  

Expression and clinical analysis of snail in 41 patients with non small cell lung cancer

作　　者：卢锐 冼磊[1] 武国煜 

机构地区：[1]广西医科大学第二附属医院心胸外科,南宁市530021

出　　处：《实用医学杂志》2017年第22期3733-3736,共4页The Journal of Practical Medicine

基　　金：广西自然科学基金面上项目(编号:2013GXNSFAA019270);广西壮族自治区教育厅高科科研项目(编号:YB2014060)

摘　　要：目的为研究snail在非小细胞肺癌(NSCLC)中表达水平差异以及snail的表达水平与NSCLC患者临床病理特征的关系。方法收集NSCLC患者术中切除的癌组织、配对癌旁组织以及因良性疾病切除的正常肺组织。采用实时免疫荧光定量PCR(qRT-PCR)法检测snail mRNA的表达及免疫组化S-P法检测snail的蛋白水平表达。结果 NSCLC组织snail mRNA及蛋白表达均明显高于配对癌旁组织(P<0.01)及正常的肺组织标本(P<0.01);snail mRNA和蛋白阳性表达与NSCLC淋巴结转移、临床分期相关(P<0.05)。结论高表达的snail可能在某种程度上促进了NSCLC的转移,snail可以作为判定NSCLC恶性程度的参考因素及预后不良的高危因素,为snail的形态学及功能学实验的深入研究和靶向治疗提供了重要基础。Objective To study the expressions of snail mRNA and protein in human non-small cell lung cancer （NSCLC） and their correlations to patient clinical features. Methods Real-time fluores-cence quantitative PCR （qRT-PCR） and immunohistochemical were used to analyze the expressions of snail in a total of 41 pairs of NSCLC tissues, 12 specimens of normal lung tissues. Results The expressions of snail mRNA in NSCLC tissues were significantly higher than the corresponding adjacent tissues （P 〈 0.01 ）. The expression rate of snail protein of NSCLC tissues （25/41） were significantly higher than that in adjacent tissues （2/41, P 〈 0.01 ）. There was no snail expression in non-cancerous lung tissues. The level of mRNA and protein in snail was associated with lymph node metastasis and clinical stage of non-small cell lung cancer （P 〈 0.05）. Conclusion The higher expression of snail may promote the metastasis of lung cancer. The expressions of snail are related to the development of human non-small cell lung cancer.

关 键 词：癌 非小细胞肺 SNAIL基因 实时荧光定量PCR 免疫组化 临床特征 

分 类 号：R734.2[医药卫生—肿瘤]