高强度聚焦超声对子宫肌瘤患者免疫功能的影响  

Inhibitory Effect of the Steroid Saponins from the Flowers of Cestrum Nocturnum,Linn(SSFCN) on Proliferation of Leukemia K562 Cells and PI3K/AKt Pathway Regulation

作　　者：弓瑜[1] 王璐[2] 

机构地区：[1]河南省郑州市妇幼保健院,450000 [2]郑州大学第一附属医院,450000

出　　处：《实用癌症杂志》2017年第12期2067-2069,2073,共4页The Practical Journal of Cancer

摘　　要：目的比较高强度聚焦超声(HIFU)和子宫肌瘤切除术(Myomectomy)对子宫肌瘤患者的免疫功能的影响。方法将子宫肌瘤患者80例,随机分为HIFU组或Myo组。于入院时和术后24 h采集静脉血样,流式细胞术检测患者CD_4^+、CD_8^+、CD_4^+/CD_8^+、NK细胞水平,酶联免疫吸附法(ELISA)检测患者血清IL-2,IL-6和IL-10水平。结果HIFU组患者恢复快,术后并发症较少,住院时间较短(P<0.05)。行HIFU术后患者CD_4^+T细胞、CD8+T细胞、CD_4^+/CD_8^+、NK细胞水平无显著变化(P>0.05);与之相反,手术切除后24 h,患者CD_4^+T细胞、CD_8^+T细胞、NK细胞水平均显著下降(P<0.05),CD_4^+/CD_8^+比例也显著下降(P<0.05)。治疗后,两组患者血清IL-6和IL-10水平均显著升高,IL-2水平显著降低;HIFU组患者IL-6和IL-10水平显著低于Myo组,Myo组患者IL-2水平明显高于HIFU组。结论HIFU对患者免疫功能影响较小,更好的免疫功能可能与组织创伤应激减少有关,并有助于减少术后并发症的发生。Objective To compare the effect of high-intensity focused ultrasound（HIFU）and conventional myomectomy on immune function in patients with uterine fibroids.Methods 80 patients with uterine fibroids were divided into the HIFU or Myo group randomly.Venous blood samples were collected 24 h before and after operation.The percentages of CD_4~＋and CD_8~＋T cells and natural killer（NK）cells were quantified by flow cytometry（FCM）.Serum levels of interleukin-2（IL-2）,IL-6 and IL-10 were determined by enzyme-linked immunosorbent assay.Results HIFU was associated with earlier recovery,fewer post-operative complications,and shorter hospital stay（P0.05）.The levels of CD_4~＋and CD_8~＋T cells and NK cells in the HIFU group were not significantly altered after treatment compared with before treatment.In contrast,the levels of these cells in the Myo group decreased significantly after conventional myomectomy（P0.05）.The CD_4~＋/CD_8~＋T cell ratios also decreased significantly（P0.05）.Serum levels of IL-6 and IL-10 increased after treatment in both groups.IL-6 and IL-10 levels were significantly lower in the HIFU group than in the Myo group（P0.05）.In contrast,IL-2 level decreased significantly in the Myo group compared to the HIFU group（P0.05）.ConclusionHIFU treatment has little influence on immune functions.Better preserved immune function may reflect a reduction in tissue trauma after HIFU treatment and has less complications.

关 键 词：高强度聚焦超声 子宫肌瘤 免疫功能 

分 类 号：R737.33[医药卫生—肿瘤]