运用交配型基因分子标记辅助蛹虫草亲和配对  

The Compatible Crosses Assisted by the Molecular Marker of the Mating Genes of Cordyceps militaris

作　　者：鲍大鹏[1] 刘敏祥[1,2] 汪滢[1] 杨瑞恒[1] 茅文俊[1] 何华奇[2] 

机构地区：[1]国家食用菌工程技术研究中心/农业部南方食用菌资源利用重点实验室/上海市农业遗传育种重点开放实验室/上海市农业科学院食用菌研究所,上海201403 [2]安徽科技学院生命科学学院,安徽凤阳233100

出　　处：《核农学报》2017年第11期2113-2120,共8页Journal of Nuclear Agricultural Sciences

基　　金：上海市科委平台建设项目(16DZ2281300)

摘　　要：为了建立一种分子标记辅助育种技术体系以增加蛹虫草杂交亲和性配对的比例,以4个蛹虫草菌株(GD1、JS2、SD2、中13)为供试亲本,通过无性孢子分离方法获得了8株单交配型的杂交亲本,根据蛹虫草交配型位点MAT-alpha和MAT-HMG中基因序列设计了分子标记并指导配制亲和性组合,最终成功获得了12个杂交菌株。在栽培试验的基础上,分析了12个杂交菌株的栽培周期、产量、活性成分含量(虫草素和腺苷)等相关性状,结果表明,蛹虫草杂交菌株存在超亲现象。本研究建立的这套以交配型基因作为分子标记辅助选育蛹虫草新品种的高效杂交育种技术体系,将有助于促进蛹虫草栽培产业的发展。To establish a molecular marker assistant breeding for increasing the ratio of compatible crosses in Cordyceps militaris, eight single-asexual-spore isolates were isolated from 4 tested strains （GD1, JS2, SD2 and Zhong 13） of C. militaris, and were mated each other according to their mating type. Twelve compatible crosses were identified by the molecular marker of mating type locus MAT-alpha and MAT-HMG. Based on the cultivation test, the cultivation period, the yield and the content of activity compounds （ cordycepin and adenosine） of the 12 hybrid strains were evaluated. The results showed that some hybrid strains displayed heterosis. The effective technique established in this study, which use the mating type genes as the molecular markers to assist the breeding of C. militaris, will be helpful for the development of cultivation of C. militaris.

关 键 词：蛹虫草 交配型基因 分子标记 杂交育种 

分 类 号：S567.35[农业科学—中草药栽培]