COX-2、MMP-9与PTEN在结肠腺癌组织中的表达及意义  

Expression and significance of COX-2,MMP-9 and PTEN in colonic adenocarcinoma tissues

作　　者：李志锋[1] 刘进忠[1] 石荣亚[1] 霍曼[1] 赵小利[1] 

机构地区：[1]河北省保定市第一医院普外科,071000

出　　处：《重庆医学》2017年第33期4676-4679,共4页Chongqing medicine

摘　　要：目的探讨环氧合酶-2(COX-2)、基质金属蛋白酶-9(MMP-9)和PTEN在结肠腺癌组织中的表达及意义。方法收集该院2014年3月至2016年5月结肠腺癌根治术后病理存档的石蜡标本72例,30例结肠腺瘤组织及30例正常结肠黏膜组织为对照。采用免疫组化SP法检测COX-2、MMP-9和PTEN的表达情况,分析COX-2、MMP-9和PTEN与结肠腺癌临床病理特征的相关性。结果 COX-2、MMP-9在结肠腺癌组织中表达阳性率明显高于结肠腺瘤组织、正常结肠黏膜组织(P<0.05),PTEN的表达阳性率明显低于结肠腺瘤组织、正常结肠黏膜组织(P<0.05)。COX-2、MMP-9和PTEN在结肠腺癌的表达与肿瘤浸润深度、有无淋巴结转移及TNM分期相关(P<0.05),与性别、年龄、肿瘤形态、肿瘤直径、分化程度无关(P>0.05)。COX-2、MMP-9与PTEN表达均呈负相关(r=-0.260、-0.282,P<0.05),COX-2与MMP-9表达呈正相关(r=0.335,P<0.05)。结论COX-2、MMP-9与PTEN的异常表达和相互作用与结肠腺癌的发生、侵袭转移密切相关,联合检测对结肠腺癌早期诊断、预后评估具有重要意义。Objective To explore the expression and significance of COX-2,MMP-9 and PTEN in colonic adenocarcinoma tissues.Methods Seventy-two cases of pathologically archived paraffin specimens after colonic adenocarcinoma radical operation in the hospital from March 2014 to May 2016 were collected,30 cases of colonic adenoma tissues and 30 cases of normal mucosal membrane tissues served as the controls.The expressions of COX-2,MMP-9 and PTEN were detected by adopting the immunohistochemical SP method.Then the correlation between COX-2,MMP-9 and PTEN with clinicopathological features of colonic adenocarcinoma was analyzed.Results The positive expression rate of COX-2 and MMP-9 in colonic adenocarcinoma tissues was significantly higher than that in colonic adenoma tissues and normal colon mucosal membrane tissues（P〈0.05）;the positive expression rate of PTEN was significantly lower than that in colonic adenoma tissues and normal colon mucosal membrane tissues（P〈0.05）.The expression of COX-2,MMP-9 and PTEN in colonic adenocarcinoma was correlated with the tumor invasive depth,lymph node metastasis and TNM staging（P〈0.05）,while had no correlation with sex,age,tumor morphology,tumor size and differentiation degree（P〈0.05）.COX-2,MMP-9 and PTEN expressions showed negative correlation（r=-0.260,-0.282,P〈0.05）,COX-2 and MMP-9 expression showed a positive correlation（r=0.335,P=0.004）.Conclusion The abnormal expression and interaction of COX-2,MMP-9 and PTEN are closely correlated with the occurrence,invasion and metastasis of colonic adenocarcinoma.Their combined detection has an important significance for early diagnosis and prognosis assessment of colonic adenocarcinoma.

关 键 词：基质金属蛋白酶-9 结肠腺癌 淋巴结转移 环氧合酶-2 PTEN 

分 类 号：R735.35[医药卫生—肿瘤]