CRISPLD2蛋白对内毒素-诱导急性呼吸窘迫综合征小鼠炎性因子表达的影响  

Effect of CRISPLD2 on the expression of inflammatory cytokine in LPS-induced ARDS mice

作　　者：刘雪峰[1] 李文放[1] 林兆奋[1] 

机构地区：[1]上海长征医院急救科,上海200003

出　　处：《海军医学杂志》2017年第6期503-505,共3页Journal of Navy Medicine

摘　　要：目的探讨CRISPLD2蛋白对内毒素-诱导急性呼吸窘迫综合征(LPS-ARDS)小鼠体内炎性因子表达的影响。方法采用数字表法将40只雄性昆明小鼠随机分5组:对照组(A组),气道内滴入0.1 mg内毒素(LPS);r CRISPLD2蛋白1组(B组)、r CRISPLD2蛋白2组(C组),小鼠尾静脉分别注射1.0、0.4 mg r CRISPLD2蛋白,6 h后气道内滴入0.1mg LPS;r CRISPLD2蛋白3组(D组)、r CRISPLD2蛋白4组(E组),予小鼠气道内滴入0.1mg LPS,6 h后予小鼠尾静脉分别注射1.0、0.4 mg r CRISPLD2蛋白。每组小鼠在LPS气道滴入后第12 h收集支气管肺泡灌洗液(BALF),离心后计数沉淀中性粒细胞,测量上清液及血标本中的肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)及IL-6。结果 B、C组BALF中的中性粒细胞计数(0.31±0.07、0.76±0.08)ng/L、TNF-α(8.88±17.37、134.63±24.44)ng/L、IL-1β(48.63±15.61、99.75±12.30)ng/L、IL-6(80.75±21.03、140.12±29.29)ng/L均明显低于A、D、E组(1.64±0.10、1.73±0.13、1.65±0.08),差异有统计学意义(P<0.05);B组以上检测值均明显低于C组,差异有统计学意义(P<0.05)。结论 CRISPLD2蛋白可下调LPS所诱导的炎症因子表达水平,并且呈现一定的剂量依赖关系。Objective To investigate the effect of CRISPLD2 protein on the expression of inflammatory cytokine in LPS-induced acute respiratory distress syndromes(ARDSs) mice.Methods Forty male Kunming mice were randomly divided into 5 groups;the control group(group A),which was given 0.1 mg LPS via the intratracheal instillation;the rCRISPLD2 group 1(group B) and the rCRISPLD2 group 2(group C),which were respectively treated with 1 mg and 0.4 mg rCRISPLD2 by tail vein,and after 6 hours rCRISPLD2 was injected intratracheally;the rCRISPLD2 group 3(group D) and the rCRISPLD2 group 4(group E),which were first given 0.1 mg LPS intratracheally and then were injected with 1 mg and 0.4 mg rCRISPLD2 by tail vein.At hour 12 after intratracheal injection of LPS in the mice of all the groups,bronchoalveolar lavage fluid(BALF) was collected.After centrifugation,the number of neutrophils was counted,and the levels of TNF-α,IL-1β and IL-6 were detected in supernatant fluid and blood samples.Results The number of neutrophils(0.31±0.07、0.76±0.08),TNF-α(8.88±17.37、134.63±24.44),IL-1β(48.63±15.61,99.75±12.30)and IL-6(80.75±21.03,140.12±29.29) in serum and BALF in groups B and C were all significantly lower than those of group A,D and E,and statistical significance could be noted,when comparisons were made between these groups(P<0.05).The detected data of group B were obviously lower than those of group C,also with statistical significance(P<0.05).Conclusion rCRISPLD2 could down-regulate the expression levels of LPS-induced inflammatory factors,and dosage dependence could be noted to a certain extent.

关 键 词：CRISPLD2蛋白 内毒素 急性呼吸窘迫综合征 炎症因子 

分 类 号：R576[医药卫生—消化系统]