猪毛蒿不同炮制方法炮制前后化学成分变化  

Variations of the Chemical Components in Different Processed Artemisia scoparia Waldst. et Kit.

作　　者：吴怀墨 袁荣贤 张霞[1] 龙澍普 李明[3] 刘华[3] 安钰[3] 陈靖[1,4] 

机构地区：[1]宁夏医科大学药学院,宁夏银川750004 [2]中药材技术服务站,宁夏盐池县科学技术局,宁夏盐池751500 [3]宁夏农林科学院荒漠化治理研究所,宁夏银川750004 [4]回医药现代化省部共建教育部重点实验室,宁夏医科大学,宁夏银川750004

出　　处：《安徽农业科学》2017年第35期111-115,共5页Journal of Anhui Agricultural Sciences

基　　金：宁夏科技攻关重点研发项目(2015BN24);宁夏回族自治区大学生创新创业计划项目“1H-NMR定量测定醋制苦豆子前后6种生物碱含量差异”

摘　　要：[目的]研究猪毛蒿不同炮制方法炮制前后水提取物、醇提取物含量、挥发性成分以及绿原酸含量差异,为寻找猪毛蒿炮制方法奠定基础.[方法]以重量法测定水提取物、醇提取物含量,GC-MS/MS测定挥发性成分,HPLC-UV测定绿原酸含量.[结果]与生品相比,晒干品的水、醇提取物含量均显著下降;烘干品水提取物含量下降,而醇提取物含量提高;阴干品无显著差异.GC-MS/MS分析不同炮制品中挥发性成分,从生品、阴干、晒干、烘干品中分别检出25、21、27、24个色谱峰,共有的组分有13个,且猪毛蒿炮制前后既有含量的改变,也有不同结构的成分出现,晒干与生品差距最大;HPLC-UV方法测定了绿原酸含量,发现炮制后均显著下降,含量从大到小依次为生品、阴干、晒干、烘干.[结论]猪毛蒿在加工过程中应尽量采用阴干方法,以防止有效成分损失.[ Objective ] The research aimed to study the difference of water extract, alcohol extract content, volatile component and chlorogen- ic acid content before and after the processing of Artemisia scoparia in different processing methods, which laid the foundation for searching the method of processing Artemisia scoparia. [Method]The gravimetric method was used to determine the water and alcohol extract content. GC- MS/MS method was applied to determine the volatile component and HPLC-UV assay was used to determine the chlorogenic acid content. [Re-sult] Compared with the crude materials, the water and alcohol extracts in the products dried in the sun decreased significantly;the content of water extract of dried product decreased, but the content of alcohol extract increased; there was no significant difference between crude and dried in the shade. GC-MS/MS analysis of volatile components in different processed products,25, 21, 27 , and 24 peaks were respectively detected in crude, dried in the shade, in the sun and in the drying oven, 13 mutual peaks were found in the four materials,and Artemisia scop-aria before and after processing both the content of the change, there were also different components of the structure, the difference between dried in the sun and crude was the biggest. Content of chlorogenic acid determined by HPLC-UV method,that found the content was decreased significantly after processing, the content in the order below crude 〉 dried in the shade 〉 dried in the sun 〉 dried in the drying oven. [ Conclu-sion ] Artemisia scoparia shall be dried in the shade during the process in order to avoid the loss of the effective components.

关 键 词：猪毛蒿 炮制方法 化学成分 HPLC-UV GC-MS/MS 

分 类 号：R283.1[医药卫生—中药学]