正交试验优化天舒胶囊超声波提取工艺  

Optimization of Ultrasonic Extraction Technology for Tianshu Capsules by Orthogonal Test

作　　者：伏东宁 李祥永[1] 王振中[2] 吴建雄[2] 倪彩莉 

机构地区：[1]连云港市中医院药学部,江苏连云港222004 [2]江苏康缘药业股份有限公司研究院,江苏连云港222004 [3]江西中医药大学中药学院,南昌330045

出　　处：《中国药房》2017年第34期4853-4856,共4页China Pharmacy

基　　金：国家“重大新药创制”--现代中药创新集群与数字制药技术平台基金资助项目(No.2013ZX09402203)

摘　　要：目的:优化天舒胶囊超声波提取工艺。方法:以天舒胶囊中川芎有效成分阿魏酸、天麻有效成分天麻素的转移率为考察指标,采用L9(34)正交设计试验,考察乙醇体积分数、乙醇用量、提取时间、超声功率等因素对转移率的影响,优化天舒胶囊超声波提取工艺并进行验证试验。结果:优化的天舒胶囊的超声波提取工艺为8倍量的70%乙醇,在超声功率350 W下提取2次,每次提取1.5 h。验证试验中,阿魏酸和天麻素转移率平均值分别为94.06%、95.02%(RSD分别为0.18%、0.47%,n=3)。结论:优化后的提取工艺方法快速、简单,结果稳定、可行,可用于天舒胶囊中有效成分的提取。OBJECTIVE：To optimize the ultrasonic extraction technology for Tianshu capsules. METHODS：Using the transfer rate of active ingredient ferulic acid in Ligusticum chuanxiong and gastrodin in Gastrodia elata of Tianshu capsules as investigation indexes,L_9（3~4）orthogonal design test was adopted to investigate the effects of ethanol volume fraction,ethanol amount,extraction time and ultrasonic power on the extraction rate. Ultrasonic extraction technology for Tianshu capsules was optimized,and verification test was carried out. RESULTS：The optimized extraction technology for Tianshu capsules was as follow as 8-fold 70% ethanol,extracting twice under 350 W,extracting 1.5 h every time. Results of verification test showed the average transfer rate was94.06% for ferulic acid（RSD=0.18%,n=3）and 95.02% for gastrodin（0.47%,n=3）. CONCLUSIONS：The optimized technology is rapid,simple,stable and feasible,and can be used for extracting the active ingredients in Tianshu capsules.

关 键 词：超声波提取 天舒胶囊 正交试验 川芎 天麻 阿魏酸 天麻素 工艺优化 含量测定 

分 类 号：R284.2[医药卫生—中药学]