高效液相色谱法同时测定蔬菜中10种植物生长调节剂残留  

Simultaneous determination of 10 plant regulators in vegetables by high performance liquid chromatography

作　　者：胡晓科[1] 文君[1] 邹海民[1] 杨晓松[1] 

机构地区：[1]成都市疾病预防控制中心,四川成都610041

出　　处：《中国卫生检验杂志》2017年第22期3218-3220,3228,共4页Chinese Journal of Health Laboratory Technology

基　　金：成都市科技局科技惠民技术研发项目(2015-HM01-00184-SF)

摘　　要：目的建立高效液相色谱仪同时检测蔬菜中噻苯隆、氯吡脲、脱落酸、6-糖基氨基嘌呤、N6-异戊烯腺嘌呤、6-苄氨基嘌呤、吲哚-3-丁酸、吲哚-3-乙酸、α-萘乙酸、α-萘乙酸甲酯10种植物生长调节剂残留量的方法。方法乙腈提取样品中的待测物,蒸发除去多余乙腈。残渣用二氯甲烷溶解,溶液过氨基柱净化,用甲醇洗脱分析物,浓缩后,以Agilent C18为分析柱,0.1%甲酸-甲醇进行梯度洗脱,紫外/荧光检测器同时检测。以保留时间定性,标准曲线外标法定量。结果 10种植物植物生长调节剂的检出限为1.0μg/kg^8.0μg/kg,线性范围0.020μg/ml^5.00μg/ml,相关系数r均>0.999,回收率为70%~104%,精密度RSD均<4.0%。结论本方法操作简便、灵敏度高、重现性好,应用于日常检测可大大降低检测成本,缩短检测周期,适合基层推广。Objective To establish the high-performance chromatographic method for simultaneous determination of ten plant regulators including thidiazuron,forchlorfenuron,abscisic acid,6-glycosyl amino purine,N6-Isoprene adenine,6-Benzyl amino purine,indole-3-butyric acid,indole-3-acetic acid,α-naphthaleneacetic acid,methyl 1-naphthylacetate in vegetables. Methods The plant regulators residues in samples were extracted by acetonitrile,and the excess acetonitrile was removed by evaporation. The residues were then dissolved in dichloromethane. The solution was cleaned up with NH2 cartridge.The analytes were eluted by methanol,and then concentrated. Chromatographic analysis was carried out on Agilent C18 column by methanol/water（ 0. 1% HCOOH） in gradient program,using both DAD and FLD as detectors. The retention time was qualitative,and the standard curve external standard method was used to quantify the retention time. Results The detection limits of 10 regulators were within 1. 0 μg/kg-8. 0 μg/kg. When the concentrations were within 0. 020 μg/ml-5. 00 μg/ml,the correlation coefficient（ r） was above 0. 999. The recoveries were within 70%-104%,the RSD of precision was less than 4. 0%. Conclusion The method is simple with good sensitivity and reproducibility. The practical application brings less detection cost and period. It is suitable for primary units to popularize.

关 键 词：高效液相色谱法 紫外-荧光检测法 植物生长调节剂 蔬菜 

分 类 号：R155.5[医药卫生—营养与食品卫生学]