可视化诺如病毒GⅡ型RT-LAMP方法在感染性腹泻疫情中的应用研究  

Application of visual detection of norovirus GⅡwith reverse transcription loop-mediated isothermal amplification in infectious diarrhea outbreaks

作　　者：谭震 李莎[2] 

机构地区：[1]长沙县食品安全检测中心,湖南长沙410100 [2]长沙市疾病预防控制中心,湖南长沙410006

出　　处：《中国卫生检验杂志》2017年第22期3236-3238,共3页Chinese Journal of Health Laboratory Technology

基　　金：湖南省卫生计生委2015年度科研计划项目(B2015-153)

摘　　要：目的探讨可视化诺如病毒(norovirus,NoV)GⅡ型逆转录环介导等温扩增(reverse transcription-loop-mediated isothermal amplification,RT-LAMP)方法在感染性腹泻疫情中的应用价值。方法利用文献报道的可视化NoV GⅡRTLAMP检测方法和RT-PCR方法分别对151份感染性腹泻疫情相关标本(80份病例粪便、43份肛拭子、16份末稍水和12份桶装水)标本进行NoV检测;评价RT-LAMP法和RT-PCR方法的一致性。结果 RT-LAMP方法检出42份感染性腹泻疫情标本NoV GⅡ型RNA阳性,阳性率为27.81%;RT-PCR方法检出37份感染性腹泻疫情标本NoV GⅡRNA阳性,阳性率为24.50%;2种方法的检测结果经统计学分析,差异无统计学意义(P>0.05)。结论可视化NoV GⅡRT-LAMP方法可用于感染性腹泻疫情的NoV GⅡRNA筛查。Objective To evaluate the application value of reverse transcription-loop-mediated isothermal amplification（ RT-LAMP） for norovirus（ No V） GⅡ in infectious diarrhea outbreaks. Methods 151 specimens from infectious diarrhea（ 80 fecal specimens,43 rectal swab specimens,16 tap water specimens and 12 bottled water specimens） were detected with the RT-LAMP assay and RT-PCR assay for visual No V GⅡ. To compare the consistency of the RT-LAMP assay and RT-PCR assay.Results No V GⅡpositive RNA was detected in 42 specimens from infectious diarrhea with RT-LAMP,and the positive rate was 27. 81%; 37 positive specimens were detected in RT-PCR assay,with the positive rate of 24. 50%; test results of the two assays were conducted for statistical analysis,with the differences not statistically significant（ P 〉0. 05）. Conclusion The visual No V GⅡwith RT-LAMP assay is potentially useful for the No V GⅡRNA screening of infectious diarrhea.

关 键 词：逆转录环介导等温扩增 NOV GⅡ 检测 

分 类 号：R725.1[医药卫生—儿科]