基于老龄大鼠的Ⅱ型糖尿病模型制备方法研究  

The Study of Developing Type Ⅱ Diabeticanimal Models Based on Aged Rats

作　　者：黄剑平[1,2] 周慧[1,2] 张裕[3] 杨子健[1,2] 汪圆圆[1,2] 李光林 

机构地区：[1]中国科学院深圳先进技术研究院,深圳518055 [2]中国科学院人机智能协同系统重点实验室,深圳518055 [3]遵义医学院珠海校区,珠海519041

出　　处：《集成技术》2017年第6期31-36,共6页Journal of Integration Technology

基　　金：深圳市基础研究布局项目(JCYJ20150529150715499)

摘　　要：为探索基于老龄大鼠建立"Ⅱ型"糖尿病模型的方法。该研究纳入12只12月龄雄性Wistar大鼠,在大鼠连续4个月自由采食高脂饲料后,给予3次腹腔注射低剂量链脲佐菌素溶液(剂量为20 mg/kg,浓度为10 mg/m L),并检测给药前后大鼠空腹血糖浓度数据,将空腹血糖浓度高于11.1 mmol/L的大鼠定为"Ⅱ型"糖尿病模型。实验结果显示,大鼠自由采食高脂饲料4个月后,体重由604.8±13.7 g增加为760.6±31.6 g(P<0.01);连续3次注射低剂量链脲佐菌素溶液后,空腹血糖浓度由5.6±0.7 mmol/L增加为22.9±2.7 mmol/L(P<0.01);继续观察2周后,大鼠空腹平均血糖浓度为17.4 mmol/L,仍显著高于糖尿病诊断标准的空腹血糖最低值11.1 mmol/L(P<0.01),糖尿病模型制备的成功率为83.3%。结果表明,连续注射3次低剂量链脲佐菌素溶液并结合高脂饲料饲养,可成功将老年Wistar大鼠诱导为"Ⅱ型"糖尿病模型。To investigate the methods of developing type II diabetic rat models, 12 male Wistar rats aged 12 months were adopted in the experiment. Firstly, the rats were naturally fed with high-fat food for four months, then the rats were weighted and their fasting blood glucose （FBG） levels were measured. Then, each rat was injected with a small dose of streptozotocin （STZ） solution （dose： 20 mg/kg, density： 10 mg/mL） for three times and their FBG levels were measured again within one week after each injection. Subsequently, the rats were considered as type II diabetic models when their FBG values were higher than 11.1 mmol/L. The results show that the weights of Wistar rats increase significantly after feeding with high fat diet for four months. Besides, the FBG levels of the rats increase from 5.6±0.7 mmol/L to 22.9±2.7 mmol/L with a significant difference （P〈0.01）. Furthermore, the averaged FBG level of the rats is significantly higher than the standard FBG level of 11.1 mmol/L and with a successful rate of 83.3%. In conclusion, the proposed method can develop type II diabetic models with Wistar rats.

关 键 词：糖尿病模型 老龄大鼠 链脲佐菌素 高脂摄食 

分 类 号：R-332[医药卫生] R587.1