小鼠白细胞介素-15基因联合RM-1前列腺癌细胞裂解产物上清共同修饰的树突状细胞疫苗制备  

Preparation of dentritic cell vaccine modified by adenovirus - mediated mouse interleukin - 15 gene and cell lysate on mouse with prostate cancer

作　　者：田子农 温端改[2] 何小舟[1] 徐仁芳[1] 许贤林[1] 侯建全[2] 

机构地区：[1]常州市第一人民医院泌尿外科,213003 [2]苏州大学附属第一医院泌尿外科,215006

出　　处：《中华实验外科杂志》2017年第12期2031-2034,共4页Chinese Journal of Experimental Surgery

基　　金：常州市卫生局科研资助项目（QN201201）;江苏省重点实验室临床免疫高技术平台培育点资助项目

摘　　要：目的探讨制备小鼠白细胞介素-15（mIL-15）基因重组腺病毒载体联合RM-1前列腺癌细胞裂解产物上清共同修饰的树突状细胞（mIL-15／lysate—DC）疫苗的可行性。方法mlL．15基因的重组腺病毒联合RM-1前列腺癌细胞裂解产物上清共同修饰树突状细胞（DC）。流式细胞术检测感染效率及DC表型。结果mlL-15基因重组腺病毒载体有效感染DC，感染效率达（77．43±1．96）％；流式细胞术结果证实mIL-15／lysate—DC组与对照组比较，其CD40[（52．50±2．36）％]、CD80[（46．53±0．91）％]、CD86[（47．10±2．59）％]、CD11c[（32．07±1．72）％]和MHC—II[（53．57±2．72）％]等表面标志显著提高，具有更强的T细胞增殖能力。结论成功制备mIL-15／lysate．DC疫苗。Objective To investigate the feasibility of preparation of dentritie cell vaccine modi- fied by adenovirus - mediated mouse intedeukin （raiL） - 15 gene and cell lysate on mouse with prostate cancer. Methods Dentritic cells （DCs） are modified by adenovirus -mediated mIL - 15 gene and cell lysate on mouse with prostate cancer. The transduction efficiency and phenotypes of DCs were analyzed by flow cytometry （FCM）. Results DCs are effectively infected by adenovirus - mediated mIL - 15 gene with a transduetion efficiency of （77.43 ± 1.96 ） % , which is confirmed by FCM. After modified by adeno- virus -mediated mIL - 15 gene and cell ]ysate on mouse with prostate cancer, the expresson of surface mol- ecules of DCs were up - regulated, inculding CD40 [ （ 52. 50 ± 2. 36） % ], CD80 [ （46. 53 ± 0. 91 ） % ], CD86 [（47.10 ± 2.59）%], CDllc [（32.07 ± 1.72）%] and major histocompatibility complex （MHC） - II[ （53.57 ±2. 72）% ]. Compared with control groups, the mIL- 15/lysate DC vaccine can stimulate the proliferation of T cells more strongly. Conclusion The mlL - 15/lysate DC vaccine has been successfully prepared and identified.

关 键 词：白细胞介素-15 前列腺癌 树突状细胞 疫苗 

分 类 号：R737.25[医药卫生—肿瘤]