七叶皂苷对人胃癌耐药细胞株SGC7901/5-氟尿嘧啶耐药性的影响及其机制  

Effect and mechanism of Escin on drug resistance of human drug resistant gastric cancer cell line SGC7901/5-fluorouracil

作　　者：杨冬野[1] 焦洋[2] 胡晓杰[1] 杜志坚 董建涛 蔡建辉[1] 

机构地区：[1]河北省人民医院普外四科,石家庄050051 [2]河北省人民医院消化科,石家庄050051

出　　处：《中华实验外科杂志》2017年第12期2135-2138,共4页Chinese Journal of Experimental Surgery

摘　　要：目的检测七叶皂苷（Escin）对胃癌耐药细胞株SGC7901/5-氟尿嘧啶（5-Fu）耐药性的影响，并探讨分子机制。方法噻唑蓝（MTT）法检测Escin（80 μg/ml）、5-Fu（20 μg/ml）单独或联合干预对细胞活性的影响。流式细胞术（FCM）检测Escin对SGC7901/5-Fu细胞凋亡的影响。实时定量聚合酶链反应（Real-time PCR）和Western blot法检测各组细胞耐药相关基因表达。结果MTT结果显示，在20、40 μg/ml的5-Fu作用后细胞活性由强到弱分别为SGC7901/5-Fu、SGC7901、GES-1（P＝0.033、0.000）。随Escin剂量增高SGC7901/5-Fu细胞活性逐渐降低（P＝0.000）。将Escin与5-Fu联合作用后SGC7901/5-Fu细胞活性在空白组[（104.38±22.83）%]、Escin组[（52.65±10.14）%]、5-Fu组[（76.26±12.84）%]、Escin＋5-Fu组[（28.42±6.48）%]依次下降（P＝0.000）。与对照组比较，Escin作用后，SGC7901/5-Fu细胞的凋亡率[（28.44±4.64）%]比对照组[（10.46±2.04）%]明显升高（P＝0.000）。Real-time PCR和Western blot结果显示，与对照组比较，Escin作用后，SGC7901/5-Fu细胞中胸腺嘧啶合成酶（TS）、胶质瘤相关癌基因1（Gil1）、生存素（Survivin）、B淋巴细胞/白血病-2（bcl-2）的表达均比对照组减弱，半胱氨酰天冬氨酸特异性蛋白酶（Caspase）-3表达比对照组增强。结论Escin能通过调节耐药相关基因的表达而减弱胃癌细胞对5-Fu的耐药性。ObjectiveTo investigate the effect and mechanism of Escin on the drug resistance of human drug resistant gastric cancer cell line SGC7901/5-fluorouracil （5-Fu）.MethodsMethyl thiazol tetrazolium （MTT） assay was applied to detect the effects of Escin （80 μg/ml） and 5-Fu （20 μg/ml） used alone or combined on cell activity. Flow cytometry （FCM） was used to examine the effect of Escin on cell apoptosis. Real-time quantitative polymerase chain reaction （Real-time PCR） and Western blotting were utilized to investigate the expression of drug-resistant genes in different groups.ResultsThe cell activity of cell lines treated with 5-Fu at the concentration of 20, 40 μg/ml was shown from high to low in the order of SGC7901/5-Fu, SGC7901 and GES-1 （P=0.000）. The activity of SGC7901/5-Fu cells decreased gradually as the dose of Escin increased （P=0.000）. Combined used of 5-Fu and Escin inhibited the activity of cells significantly, and cell activity from high to low was shown in the order of control group [（104.38±22.83）%], Escin group [（52.65±10.14）%], 5-Fu group [（76.26±12.84）%], and Escin＋ 5-Fu group [（28.42±6.48）%] （P=0.000）. The SGC7901/5-Fu cells exhibited increased apoptosis rate [（28.44±4.64）%] after Escin intervention as compared with control group [（10.46±2.04）%] （P=0.000）. The expression of thymidylate synthase （TS）, glioma-associated oncogene homolog 1 （Gil1）, Survivin, and B cell lymphoma/leukemia-2 （bcl-2） decreased, but cysteinyl aspartate-specific protease-3 （Caspase-3） increased significantly in SGC7901/5-Fu cells after Escin intervention.ConclusionEscin could alleviate the drug resistance of SGC7901/5-Fu cells to 5-Fu by regulating drug resistance related genes.

关 键 词：胃癌 七叶皂苷 耐药性 5-氟尿嘧啶 

分 类 号：R735.2[医药卫生—肿瘤]