染色体核型分析和荧光原位杂交技术用于产前诊断的价值研究  

Study on the value of karyotype analysis and fluorescence in situ hybridization in prenatal diagnosis

作　　者：禤洁甜[1] 梁彩红[1] 伍启康[1] 

机构地区：[1]广东省佛山市第一人民医院检验科,广东佛山528000

出　　处：《吉林医学》2017年第12期2220-2221,共2页Jilin Medical Journal

摘　　要：目的:探究染色体核型分析和荧光原位杂交技术用于产前诊断的价值。方法:选取行羊水穿刺检查的531例产妇的羊水样本作为研究资料,随机分为对照组(260例)和观察组(271例)。对照组羊水样本行染色体核型分析,观察组行荧光原位杂交分析,统计两组受试者染色体核型分析和荧光原位杂交分析结果,对比两组诊断成功率、异常核型检出率及两组检测效率。结果:观察组羊水样本诊断成功率高达100.00%,但与对照组(99.62%)比较,差异无统计学意义(P>0.05)。染色体核型分析方面,对照组羊水样本异常染色体核型检出率(8.86%)显著高于观察组(2.95%),组间差异有统计学意义(P<0.05)。观察组获取样本至出具报告用时(2.11±1.03)d,显著少于对照组(18.12±4.26)d,组间差异有统计学意义(P<0.05)。结论:染色体核型分析和荧光原位杂交技术用于产前诊断的优势各有千秋,临床可根据孕妇的具体情况选择不同的诊断方法,或两者结合使用。Objective To explore the value of karyotype analysis and fluorescence in situ hybridization in prenatal diagnosis. Method Amniotic fluid samples from 531 women who underwent amniocentesis were selected as research data and randomly divided into control group（260 cases） and observation group（271 cases）. The control group of the karyotype analysis of amniotic fluid,observation group for analysis of fluorescence in situ hybridization,statistics of two groups of subjects of karyotype analysis and fluorescence in situ hybridization analysis results,comparing the two groups of success rate of diagnosis,the detection rate of abnormal karyotype and two groups of detection efficiency. Results The success rate of amniotic fluid samples in the observation group was as high as 100. 00%,but there was no significant difference between the control group（99. 62%） and the control group（P〈0. 05）. In karyotype analysis,the detection rate of abnormal karyotype in amniotic fluid samples of control group（8. 86%） was significantly higher than that of observation group（2. 95%）,and the difference was statistically significant between groups（P〈0. 05）. In the observation group,when the sample was issued to the report（2. 11 ± 1. 03） d,it was significantly less than that of the control group（18. 12 ± 4. 26） d,the difference was statistically significant between groups（P〈0. 05）. Conclusion The advantages of karyotype analysis and fluorescence in situ hybridization in prenatal diagnosis are different,and different diagnostic methods can be chosen according to the specific conditions of pregnant women,or the combination of the two methods.

关 键 词：荧光原位杂交技术 染色体核型分析 产前诊断 临床价值 

分 类 号：R440[医药卫生—诊断学] R714.5[医药卫生—临床医学]