FOXP3在肝癌组织中的表达及其对抗肿瘤免疫应答的影响  

Expression of FOXP3 in hepatocellular carcinoma and its influence on tumor immune response

作　　者：王莉 王玉同 张斌 姚翠娥 王萌 

机构地区：[1]空军军医大学附属西京医院急诊科,陕西西安710032 [2]西安医学院临床医学系,陕西西安710021

出　　处：《临床和实验医学杂志》2017年第24期2448-2451,共4页Journal of Clinical and Experimental Medicine

摘　　要：目的探讨FOXP3在肝癌组织中的表达及其对抗肿瘤免疫应答的影响。方法收集2014年9月至2016年9月间接受手术治疗的原发性肝癌患者86例作为观察组,健康人50例作为正常对照组。采用荧光定量PCR法检测观察组肝癌组织、癌旁组织中FOXP3 mRNA表达量;采用流式细胞仪检测观察组、正常对照组外周血中Th17/Treg细胞比率,采用酶联免疫吸附法测定Th1/Th2细胞因子含量,采用单向琼脂扩散法测定免疫球蛋白含量。采用Pearson检验评估肝癌组织FOXP3 mRNA表达量与抗肿瘤免疫的相关关系。结果肝癌组织中FOXP3 mRNA表达量显著高于癌旁组织(P<0.05);观察组患者外周血中Th17细胞比率、Th17/Treg均显著低于正常对照组,Treg细胞比率高于正常对照组;外周血中Th1细胞因子干扰素γ(IFN-γ)、白介素-12p40(IL-12p40)的含量低于正常对照组,Th2细胞因子白介素4(IL-4)、白介素6(IL-6)的含量高于正常对照组;外周血中免疫球蛋白M(Ig M)、免疫球蛋白G(Ig G)、免疫球蛋白A(Ig A)的含量高于正常对照组(P<0.05)。结论 FOXP3在肝癌组织中高表达,可直接影响患者的抗肿瘤免疫状态。Objective To study the expression of FOXP3 in hepatocellular carcinoma and its influence on tumor immune response. Methods 86 cases of primary liver cancer patients who underwent surgical treatment in our hospital during September 2014 to September 2016 were selected as observation group,and 50 cases of healthy people were served as normal control group. The expression of FOXP3 mRNA was detected byfluorescence quantitative PCR method of observation group in hepatocellular carcinoma and pericarcinomatous tissues; flow cytometry was used to detect the observation group and normal control group patients’ percentage of Th17/Treg cells in peripheral blood,Th1/Th2 cytokines contents were measured by ELISA,agar diffusion method was used to determine the contents of immunoglobulin. Pearson test was used to evaluate the correlation between the expression of FOXP3 mRNA and antitumor immunity in HCC. Results Expression of FOXP3 mRNA in hepatocellular carcinoma tissues of observation group was significantly higher than that in paracancerous tissue（ P 〈 0. 05）; percentage of Th17 cells and the ratio of Th17/Treg in observation group in peripheral blood were significantly higher than those in normal control group,proportion of Treg cells was lower than that in normal control group; the contents of Th1 cytokines such as IL-12 p40,IFN-γin observation group in peripheral blood were lower than those in normal control group,contents of Th2 cytokines such as IL-4,IL-6 were higher than those in normal control group; the contents of IgM,IgG,IgA in peripheral blood in observation group were higher than those in normal control group（ P 〈 0. 05）. Conclusion FOXP3 is highly expressed in liver cancer tissues and it can directly affect the anti-tumor immune status of the patients.

关 键 词：肝癌 FOXP3 抗肿瘤免疫 

分 类 号：R735.7[医药卫生—肿瘤]