白藜芦醇对大鼠血液高凝态的抑制作用及其与NF-κB表达的关系  

Inhibitory effect of resveratrol on the hypercoagulable state in rats and its relationship with the expression of NF-κB

作　　者：王立文[1] 沈晓洁[1] 吴倩 

机构地区：[1]无锡卫生高等职业技术学校护理系,江苏无锡214028 [2]中国药科大学药学院药理系,南京210009

出　　处：《中国比较医学杂志》2017年第12期79-83,共5页Chinese Journal of Comparative Medicine

基　　金：无锡市医院管理中心科研项目(YGZXM1533)

摘　　要：目的研究白藜芦醇对于大鼠血液高凝态形成的影响及其与NF-κB表达的关系。方法 SD大鼠分空白组、模型组、白藜芦醇高剂量组(60 mg/kg)、白藜芦醇低剂量组(30 mg/kg)和阿司匹林组(10 mg/kg),连续灌胃给药7 d,采用肾上腺素联合冰浴法和凝血酶法建立高凝态大鼠模型,采集血样测凝血酶原时间(prothrombin time,PT)、活化部分凝血活酶时间(activated partial thromboplastin time,APTT)及全血粘度;收集血管内皮细胞,采用蛋白印迹法测NF-κB表达情况。结果空白组、白藜芦醇高剂量组和阿司匹林组较模型组PT、APTT显著延长(P<0.05),血液粘度显著降低(P<0.05);白藜芦醇高剂量组血管内皮细胞NF-κB表达较模型组明显降低(P<0.05)。结论高剂量白藜芦醇(60 mg/kg)可抑制大鼠血液高凝态形成,这种作用可能与其降低NF-κB的表达有关。Objective The topic of this study was to study the inhibitory effect of resveratrol on hypercoagulable state in rats and its relationship with the expression of NF-κB. Methods Rats were divided into control and model groups,and resveratrol（ high dose,60 mg/kg）,resveratrol（ low dose,30 mg/kg） and aspirin（ 10 mg/kg） were given by intragastric administration for 7 d. Then,the rats were treated by adrenaline with ice bath or thrombin to generate hypercoagulable state. Blood samples were collected to test the prothrombin time（ PT）,activated partial thromboplastin time（ APTT） and blood viscosity. Vascular endothelial cells were collected to detect the expression of NF-κB by western blotting. Results PT and APTT values of rats in the control,resveratrol（ high dose） and aspirin groups were significantly increased than the model group（ P〈0. 05）. Blood viscosity of rats in the control,resveratrol（ high dose） and aspirin groups was significantly lower than the model group（ P〈0. 05）. The expression of NF-κB was decreased than that of the model group（ P〈0. 05）. Conclusions Resveratrol in high dose（ 60 mg/kg） can inhibit hypercoagulable state of rat,which may be related with its effect on decreasing the expression of NF-κB.

关 键 词：白藜芦醇 高凝态 NF-ΚB 大鼠 

分 类 号：R-33[医药卫生]