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作 者:刘发生[1,2] 王荣春[1] 郭敬兰[1] 陈艳莉 夏青 孔浩天 冯润良[3] 韩利文[1] 张爱平[2] 刘可春[1]
机构地区:[1]山东省科学院生物研究所,山东省科学院药物筛选技术重点实验室,山东济南250014 [2]山西医科大学药学院,山西太原030001 [3]济南大学,山东济南250014
出 处:《山东科学》2017年第6期29-34,共6页Shandong Science
基 金:山东省重点研发计划(2016GSF121009,2016GGC01089);山东省自然科学基金(ZR2016YL009);国家海洋公益性行业科研专项(201505030)
摘 要:采用正常发育48 hpf的转基因斑马鱼为实验动物,以氯霉素处理引起免疫抑制,分别加入25.0~200.0 mg/L的芦笋多糖、6.25~100.00 mg/L的芦笋皂苷,处理24 h,观察各组斑马鱼的发育情况,拍照并统计斑马鱼体内中性粒细胞的数量。结果表明,氯霉素处理斑马鱼后,斑马鱼体内中性粒细胞数量减少,芦笋多糖在浓度为50.0~200.0 mg/L范围内对斑马鱼体内中性粒细胞数量减少具有明显改善作用,且具有明显的剂量依赖效应;芦笋皂苷在实验剂量范围内均显示出显著的改善作用。实验结果显示,芦笋的有效成分芦笋多糖、芦笋皂苷对斑马鱼免疫功能具有明显的调节作用。In this experiment, 48 hpf (hour post fertilization) healthy transgenic zebrafish Tg( lyz: EGFP) was used as animal model. Firstly, immune suppression of zebrafish embryos was induced by chloramphenicol treatment. Then those zebrafish embryos were treated with 25.0 - 200.0 mg/L asparagus polysaccharide and 6.25 - 100.00 mg/L asparagus saponins for 24 h, respectively. Finally, the development of zebrafish in each group was observed under the microscope, and the number of neutrophils in zebrafish was recorded and counted to evaluate their immune-regulatory effects. The results showed that the numbers of neutrophils in 572hpf zebrafish trunk area decreased in chloramphenicol-induced group. Asparagus polysaccharide group(50.0-200.0 mg/L) showed a significant immunological enhancement effect with dose- dependent manner. Asparagus saponins could also significantly improve chloramphenicol-induced immunosuppressive effects within the experimental dose range. In this study, This study showed that the active ingredients of asparagus, such as polysaccharide and asparagus saponin, had obvious regulation effects on the immune function of zebrafish, which provided the scientific basis for further development of asparagus.
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