吉林省部分地区羊泰勒虫病流行病学调查及分类鉴定  

Epidemiological survey and identification of theileriosis in goats and sheep in Jilin Province

作　　者：田万年 薛书江[3] 贾立军[3] 张守发[3] 李国江[1,2] 

机构地区：[1]吉林农业科技学院动物科技学院,吉林吉林132101 [2]吉林农业科技学院预防兽医学吉林省重点实验室,吉林吉林132101 [3]延边大学农学院,吉林延吉133002

出　　处：《中国兽医杂志》2017年第10期13-16,共4页Chinese Journal of Veterinary Medicine

基　　金：吉林省科技发展计划项目(20150623004TC);吉林省重点学科培育项目(吉农院合字[2015]第x030号);吉林农业科技学院种子基金项目(吉农院合字[2014]第Z07号)

摘　　要：为了解吉林省部分地区羊泰勒虫病的流行情况,试验采用PCR方法对采自吉林省部分地区羊血液样本235份进行羊泰勒虫检测,并对部分阳性样本进行测序分析,建立系统发育树。结果显示,羊泰勒虫阳性率为28.08%,为吕氏泰勒虫单独感染,没有检测到尤氏泰勒虫和绵羊泰勒虫感染。系统发育树分析表明,吉林省吕氏泰勒虫与青海分离株分布于同一分支,亲缘关系最近,与绵羊泰勒虫、尤氏泰勒虫亲缘关系较远。此次调查为吉林省羊泰勒虫病的综合防治提供了理论依据。In order to study the prevalence of theileria parasites in ovine infections in sheep. A total of 235 peripheral hlood samples of sheep were collected from Jilin and detected by microscopic examination and PCR. The 18S rRNA gene was cloned from part of positive samples and used for phylogenetical analysis. As a resuh, Theileriosis in goats and sheep was positive in 28.08% of the samples. Phylogenetic tree analysis confirmed Theileria spp. was most closely related to Tlwileria luwenshuni Qinghai strains, but more distantly related to Theileria uilenbergi and Theileria ovis. The resuhs provide important information for prevention and control of Pirnplasma of Cattle and Sheep in the region.

关 键 词：羊泰勒虫病 流行病学调查 聚合酶链反应 18S RRNA 系统发育树 

分 类 号：S852.72[农业科学—基础兽医学]