山葡萄酒MLF乳杆菌最佳培养基的筛选  

Screening of the Best Medium of MLF Lactobacillus

作　　者：刘东旭 高鹏飞 丁玉萍 薛秀珍 

机构地区：[1]佳木斯大学生命科学学院,应用微生物研究所,黑龙江佳木斯154007

出　　处：《佳木斯大学学报（自然科学版）》2017年第6期996-1000,共5页Journal of Jiamusi University：Natural Science Edition

基　　金：黑龙江省教育厅自然科学研究项目(2016-KYYWF-0612);佳木斯大学校长创新创业基金项目(xzyf2016-29);佳木斯大学研究生科技创新项目(YZ2016_009)

摘　　要：以OD_(600)值作为评价指标,对MRS和改良M17培养基进行比较选择出基础培养基,在基础培养基中添加不同的果汁探究不同果汁对MLF乳杆菌的影响;采用单因素和正交试验对果汁加量、碳源、总氮源和盐液A进行优化,得出最佳组合为:大豆蛋白胨为5.4g/L,胰蛋白胨为5.4g/L,牛肉蛋白胨为3.6g/L,酵母浸粉为3.6g/L,K_2HPO_4为0.7g/L,抗坏血酸为0.5g/L,盐液A为2g/L,甘油为8g/L,乳糖为8g/L,葡萄汁为130g/L。此培养基培养MLF乳杆菌,其培养液的OD_(600)值比改良M17培养基高大约32.2%。The test used OD600 value as the evaluation index, we elected better medium with comparingthe modified M17 medium to MRS medium. Adding different juice is to explore the effect of different juice onlactobacillus. By single factor and orthogonal, it could be optimized that involved the content of juice, C source,N source and A salt solution. It could be the best optimum combination of culture medium ： soya peptone 5.4g/L, tryptone 5.4g/L, beefpeptone 3.6g/L, yeast extract powder 3.6g/L, potassium hydrogen phosphate 0.7g/L, L- ascorbic acid 0.05g/L, the salt solution A 2g/L, glycerol 8g_/L, lactose 8g/L, grape juice 130g/L. Thismedium cultivates MLF lactobacillus, OD600 values of the culture medium about 32.2 % is higher than the im-proved M17 medium.

关 键 词：山葡萄酒 苹果酸-乳酸发酵菌 培养基 筛选 

分 类 号：Q93[生物学—微生物学]