几种色素抑制过氧亚硝酸根损伤酪氨酸能力的对比  

Comparison of Several Pigments Inhibit Peroxynitrite Damaging Tyrosine Study on the Effect of Several Pigments on Tyrosine Damage Induced by Peroxynitrite

作　　者：张辰暘 魏安琪[1] 王妍[1] 谢子奇[1] 罗云敬[1] 

机构地区：[1]北京工业大学生命科学与生物工程学院,中国北京100124

出　　处：《湖南师范大学自然科学学报》2017年第6期34-39,70,共7页Journal of Natural Science of Hunan Normal University

基　　金：国家科技支撑计划资助项目(2015BAK44B00)

摘　　要：过氧亚硝酸根可以通过强氧化性及强硝化性损伤酪氨酸,从而损害人体健康.姜黄素、原花青素、槲皮素和柚皮素具有抗氧化、抗硝化性,但其自身颜色在研究酪氨酸硝化损伤的常规方法中易造成干扰.为测定色素抑制3-硝基酪氨酸生成的能力,使用高效液相色谱-二极管阵列检测器(HPLC-PDAD,High Performance Liquid Chromatography-Photodiode Array Detector)法对硝化损伤体系成分进行分离,使用荧光光谱法测定色素抑制酪氨酸二聚体生成能力,邻苯三酚自氧化法测定超氧根阴离子抑制能力.结果表明3-硝基酪氨酸抑制率从大到小为姜黄素、原花青素、柚皮素、槲皮素、V_C;酪氨酸二聚体抑制率从大到小为原花青素、姜黄素、柚皮素、槲皮素、V_C;邻苯三酚自氧化抑制率从大到小为姜黄素、槲皮素、柚皮素、V_C,原花青素无明显影响.分析表明4种色素成分抗氧化、抗硝化能力与其结构密切相关.Peroxynitrite has strong oxidized and nitrified ability which can damage tyrosine and do harm to hu- man health. Curcumin, proanthocyanidins, quercetin and naringenin are resistant to oxidation and nitrification. However, they are easily disturbed tyrosine damage detection in conventional methods. In this paper, the components of the nitrified injury, system were separated and the ability of the pigments to inhibit 3-nitrotyrosine was determined by using HPLC-DAD. The inhibitory rates of dimer-tyrosine and the O2- from pyrogallol autoxidation were determined by fluorescence spectroscopy. The results showed 3-nitrotyrosine inhibition rate ： Curcumin 〉 Proantho- cyanidins 〉 Naringenin 〉 Quercetin; Dimer-tyrosine inhibition rate： Proanthocyanidins 〉 Curcumin 〉 Naringenin 〉 Quercetin; pyrogallol autoxidation inhibition rate： Curcumin 〉 Naringenin 〉 Quercetin, while proanthocyanidins have no significant effect. The results showed that the anti-oxidized and anti-nitrified ability of four pigments components were closely related to their structures.

关 键 词：高效液相色谱-二极管阵列检测器 荧光光谱法 过氧亚硝酸根 酪氨酸损伤 黄酮类色素 

分 类 号：Q55[生物学—生物化学]