小檗碱减轻幽门螺杆菌诱导的人胃黏膜上皮细胞损伤  

Effect of berberine on Helicobacter pylori-induced human gastric epithelial cells injury

作　　者：陈宁南[1] 万强[2] 

机构地区：[1]江西省人民医院急诊科,江西南昌330006 [2]江西中医药大学附属医院心血管病科,江西南昌330006

出　　处：《中国病理生理杂志》2017年第12期2283-2286,2292,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81660770);江西省自然科学基金资助项目(No.20161BAB215256);江西省卫生计生委科技计划(No.20171105);江西省卫生计生委中医药科研课题(No.2016A083)

摘　　要：目的:探讨小檗碱(Ber)对幽门螺杆菌(Hp)诱导的人胃黏膜上皮细胞GES-1损伤的保护作用及机制。方法:采用Hp感染GES-1细胞,加入小檗碱(5,10和20μmol/L)和细胞外调节蛋白激酶(ERK1/2)抑制剂PD98059(20μmol/L)共培养。MTT法检测细胞活力,流式细胞术检测细胞凋亡,ELISA法检测细胞白细胞介素(IL)-1β及IL-8的水平,比色法检测细胞乳酸脱氢酶(LDH)活性并以Western blot法检测细胞Bax、Bcl-2、p-ERK1/2的蛋白水平。结果:与对照组比较,Hp可抑制GES-1细胞活力、促进GES-1细胞凋亡、诱导GES-1细胞分泌IL-1β和IL-8、增加LDH活性、增加GES-1细胞内p-ERK1/2及Bax蛋白水平并降低Bcl-2蛋白水平,差异均有统计学显著性(P<0.05);与Hp感染组比较,中、高剂量小檗碱均可逆转Hp对GES-1细胞活力、细胞凋亡、细胞IL-1β及IL-8分泌、LDH活性和GES-1细胞内p-ERK1/2、Bax及Bcl-2蛋白水平的影响,差异均有统计学显著性(P<0.05);与高剂量小檗碱组比较,PD98059联合小檗碱可进一步逆转Hp对GES-1细胞上述指标的影响,差异均有统计学显著性(P<0.05)。结论:小檗碱可通过抗炎、增加细胞活力和抗凋亡来减轻Hp诱导的GES-1细胞损伤,其机制可能与抑制ERK1/2通路有关。AIM ： To investigate the effect of berberine （ Ber） on Helicobacter pylori （ Hp） -induced human gastric epithelial cells （ GES-1） injury and the underlying mechanism. METHODS： Berberine （5 , 10 and 20 jjimol/L） and PD98059 （20 jjimol/L） , a selective inhibitor of extracellular regulated protein kinases （ERK）l/2 signaling pathway, were added to Hp-infected GES-1 cells. The cell activity and apoptosis, the levels of interleukin （IL）-lp and IL-8, lactic dehydrogenase （LDH） activity and the protein levels of Bax, Bcl-2 and p-ERKl/2 in the GES-1 cells were determined by MTT assay, flow cytometry, ELISA, colorimetry and Western blot, respectively. RESULTS： Compared with control group, Hp significantly inhibited the cell activity, increased the apoptotic rate, LDH activity, IL-1 p and IL-8 levels, the Bax and p-ERKl/2 protein levels but decreased the Bcl-2 protein level in GES-1 cells （P 〈0. 05）. However, these effects of Hp were reversed by berberine at medium-dose and high-dose, as compared with the Hp-infected GES-1 cells （P 〈 0.05）. Moreover, the protective effects of berberine were significantly enhanced by the co-incubation of berberine with PD98059, as compared with the berberine at higher dose （P 〈0. 05） . CONCLUSION： Berberine may attenuate Hp-in-duced human gastric epithelial GES-1 cells injury by anti-inflammation, promoting cell growth and anti-apoptosis via the inhibition of ERK1/2 signaling pathway.

关 键 词：小檗碱 幽门螺杆菌 细胞外调节蛋白激酶1/2 人胃黏膜上皮细胞 

分 类 号：R0[医药卫生] R363