长链非编码RNA PVT1在神经胶质瘤组织中的表达及临床意义  

Expression of long non-coding RNA PVT1 in brain glioma and its clinical significance

作　　者：赵东 孙虎[1] 龚杰[1] 石晓勇[1] 陈钟樑 陈亮[1] 刘伟贤[1] 汤朱骁 麻江春 聂德康 

机构地区：[1]浙江医院神经外科,杭州310013 [2]盐城市第一人民医院神经外科

出　　处：《浙江医学》2017年第23期2106-2109,共4页Zhejiang Medical Journal

基　　金：浙江省中医药科技计划项目(2016ZQ004);浙江省医药卫生科技计划科研基金项目(2017KY179)

摘　　要：目的探讨长链非编码RNA(LncRNA)浆细胞瘤转化迁移基因1(PVT1)在神经胶质瘤中的表达及临床意义。方法采用qRT-PCR方法检测并比较53例神经胶质瘤组织(观察组)及6例正常脑组织(对照组)中LncRNA PVT1的表达量。根据神经胶质瘤组织中LncRNA PVT1的中位表达量分为高表达组与低表达组,分析LncRNA PVT1表达量与临床病理因素的关系。采用Kaplan-Meier法绘制不同LncRNA PVT1表达量的神经胶质瘤患者生存曲线,并分析LncRNA PVT1表达量与神经胶质瘤患者预后的关系。结果观察组LncRNA PVT1的表达量明显高于对照组(P<0.01)。其中高级别胶质瘤(WHOⅢ~Ⅳ级)的LncRNA PVT1表达量明显高于低级别胶质瘤(WHOⅠ~Ⅱ级)(P<0.01)。LncRNA PVT1表达量与神经胶质瘤WHO分级密切相关(P<0.01),与患者年龄、性别、Karnofsky功能状态评分(KPS)评分、肿瘤大小等均无关(均P>0.05)。经log-rank检验,高表达组患者预后较低表达组差,差异有统计学意义(P<0.01)。结论LncRNA PVT1在神经胶质瘤组织中高表达,表达量与神经胶质瘤WHO分级及患者预后密切相关;LncRNAObjective To investigate the expression of long-coding RNA(lncRNA) of plasmacytoma variant translocation gene 1(PVT1) in glioma and its clinical significance. Methods qRT-PCR was applied to detect the expression of lncRNA PVT1 in 53 specimens of glioma(glioma group) and 6 specimens of normal brain tissues(control group). The survival curves were generated with Kaplan-Meier method. The correlation between the expression of lncRNA PVT1 and clinicopathological features,prognosis of patients was analyzed. Results The expression of lncRNA PVT1 was significantly up-regulated in glioma group compared with that the control group(P<0.01). The expression of lncRNA PVT1 in high-grade neuroglioma(WHO III-IVgrade)was significantly higher than that in low-grade neuroglioma(WHO I-II grade)(P<0.01). The expression of lncRNA PVT1 was significantly associated with the WHO grading of glioma(P<0.01), but not with age, gender, Karnofsky Performance Status(KPS)score and tumor size(All P >0.05). Log-rank test revealed that patients with high expression of lncRNA PVT1 was significantly associated with worse prognosis compared with those with low expression(P<0.01). Conclusion LncRNA PVT1 is up-regulated in neuroglioma tissues. The expression of lncRNA PVT1 is closely associated with WHO grading system and prognosis of patients, indicating that lncRNA PVT1 may serve as a novel molecular biomarker for determination of the malignant degree and prognosis of neuroglioma.

关 键 词：神经胶质瘤 长链非编码RNA 浆细胞瘤转化迁移基因1 预后 

分 类 号：R739.4[医药卫生—肿瘤]