ARHⅠ对胃癌细胞TRAIL凋亡敏感性的影响  

Effects of aplysia ras homolog Ⅰ on the apoptotic sensitivity to tumor necrosis factor-related apoptosis-inducing ligand in gastric cancer cells

作　　者：唐海灵 郭汉青 张彦亭 闫媛 庄坤[1] 张欣[1] 

机构地区：[1]西安交通大学附属西安市中心医院消化内科,西安710003

出　　处：《山西医科大学学报》2017年第12期1241-1244,共4页Journal of Shanxi Medical University

基　　金：国家自然科学基金资助项目(81502084)

摘　　要：目的探讨ARHⅠ对TRAIL诱导胃癌细胞BGC-823和MKN-28凋亡敏感性的影响。方法以稳定转染p IRES2-EGFP、p IRES2-EGFP-ARHⅠ质粒载体的BGC-823胃癌细胞系和稳定转染p U6、p U6-si ARHⅠ质粒载体的MKN-28胃癌细胞系为模型,以200 ng/ml的重组人可溶性TRAIL作用于细胞24 h,采用流式细胞术检测细胞凋亡,Western blot检测Bax、Bcl-2、活化caspase-3的蛋白表达。结果过表达ARHⅠ显著增强了TRAIL诱导的BGC-823胃癌细胞凋亡,凋亡率由(6.85±1.77)%增加至(28.14±3.29)%(P<0.01),而下调ARHⅠ表达显著抑制了TRAIL诱导的MKN-28胃癌细胞凋亡,凋亡率由(35.02±3.89)%降低至(16.27±2.64)%(P<0.01);过表达ARHⅠ能够提高Bax表达2.37±0.18倍(P<0.01)、降低Bcl-2表达0.54±0.06倍(P<0.01),而下调ARHⅠ表达能够降低Bax表达0.45±0.08倍(P<0.01)、提高Bcl-2表达1.92±0.15倍(P<0.01)。结论调控ARHⅠ表达能够影响胃癌细胞BGC-823和MKN-28对TRAIL诱导凋亡的敏感性。Objective To investigate the effects of aplysia ras homolog Ⅰ（ ARHⅠ） on the apoptotic sensitivity to tumor necrosis factor-related apoptosis-inducing ligand（ TRAIL） in BGC-823 and MKN-28 gastric cancer cells. Methods BGC-823 gastric cancer cells were stably transfected with plasmid vector p IRES2-EGFP or p IRES2-EGFP-ARH Ⅰ,and MKN-28 gastric cancer cells were stably transfected with plasmid vector p U6 or p U6-si ARHⅠ. These stable cell lines were stimulated by 200 ng/ml recombinant human soluble TRAIL for 24 h. Flow cytometry was used to detect cell apoptosis and Western blot was used to detect the expression of Bax,Bcl-2 and activated caspase-3 protein. Results ARHⅠ overexpression significantly enhanced TRAIL-induced BGC-823 gastric cancer cell apoptosis,and the apoptotic rate increased from（ 6. 85 ± 1. 77） % to（ 28. 14 ± 3. 29） %（ P〈0. 01）. ARHⅠ down-regulation significantly inhibited TRAIL-induced MKN-28 gastric cancer cell apoptosis,and the apoptotic rate decreased from（ 35. 02 ± 3. 89） % to（ 16. 27 ± 2. 64） %（ P〈0. 01）. ARHⅠ overexpression increased Bax expression by 2. 37 ± 0. 18 times（ P〈0. 01） and decreased Bcl-2 expression by 0. 54 ± 0. 06 times（ P〈0. 01）,while ARHⅠ down-regulation decreased Bax expression by 0. 45 ± 0. 08 times（ P〈0. 01） and increased Bcl-2 expression by 1. 92 ± 0. 15 times（ P〈0. 01）. Conclusion Regulation of ARHⅠ expression can affect the apoptotic sensitivity of BGC-823 and MKN-28 gastric cancer cells to TRAIL.

关 键 词：胃癌 抑癌基因 ARHⅠ TRAIL 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤]