流产胎猪鲍曼不动杆菌的分离鉴定  

Isolation and identification of Acinetobacter baumannii from abortion fetal pigs

作　　者：高国强[1] 李树东[1] 王珊珊[1] 刘欢欢 周玉龙[1] 侯喜林[1] 

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《中国兽医科学》2017年第12期1577-1582,共6页Chinese Veterinary Science

摘　　要：为确诊黑龙江省某种猪场母猪发生的严重流产的病原,选择2头刚流产的胎儿剖检并无菌采取病料,结果从胎儿的肝、脾和肺组织中检测出PRV和PRRSV核酸阳性,同时还分离到1株革兰阴性细菌,对其理化特性进行鉴定,并对其进行16S r RNA测序分析。结果显示,该菌与鲍曼不动杆菌理化特性相符,并与菌株DENMARK 1O的同源性为99.2%,所以确定该菌为鲍曼不动杆菌,并命名为HLJ-1。In order to diagnose a severe miscarriage in sows in a pig farm in Heilongjiang Province, pathologic materials from 2 abortion fetal pigs were collected .The nucleic acid of PRVand PRRSV was detected in the liver,spleen and lung. At the same time,gram-negative bacteria were isolated. The physical and chemical characteristics were identified and the16S rRNA sequences were analysed. The results showed that the physicochemical properties were consistent with that of Acinetobacter baumannii and its homology with the strain DENMARK 10 from OenBank was up to 99.2% .So,the isolated strain was confirmed as Acinetobacter baumannii,named HLJ-1.

关 键 词：鲍曼不动杆菌 16S rRNA 细菌分离 流产胎儿 生化鉴定 系统进化分析 

分 类 号：S852.611[农业科学—基础兽医学]