BDNF对全脑放疗后NFAT3／c4介导的信号通路相关因子作用研究  

Effect of brain-derived neurotrophic factor on the NFAT3/c4 signaling pathway after whole brain radiotherapy

作　　者：徐美玲[1] 张军军[1] 张奇贤[1] 吴昊昊[1] 范秋虹[1] 田野[1] 

机构地区：[1]苏州大学附属第二医院放射治疗科苏州大学放射肿瘤治疗学研究所苏州市肿瘤放射治疗学重点实验室,215004

出　　处：《中华放射肿瘤学杂志》2017年第12期1438-1442,共5页Chinese Journal of Radiation Oncology

基　　金：国家自然科学基金（81372411,81172128）;江苏省临床医学科技专项资助项目（BL2014040）;苏州市科技发展计划项目（SZS201509）

摘　　要：目的 观察不同剂量电离辐射对NFAT3/c4信号通路表达的影响以及外源性的BDNF对该通路的改善作用。方法 选用1个月龄SD大鼠分成4个组分别接受单次0、2、10、20 Gy照射,照后3 d进行双侧海马立体定向注射外源性的BDNF。后取脑部海马组织进行蛋白印迹法及PCR技术观察NFAT3/c4相关蛋白表达情况。结果 蛋白印迹法及PCR结果表明电离辐射后NFAT3/c4的表达水平明显下降并呈剂量及时间依赖性。IR＋BDNF组较单纯照射组相比,随着照射剂量增加及时间延长NFAT3/c4和CaN表达水平明显升高。结论 全脑放疗抑制了CaN/NFAT3/c4信号通路,外源性BDNF能促进NFAT依赖的转录,从而改善认知功能的下降。Objective To explore the effects of various radiation doses on the NFAT3/c4 signaling pathway and the improvement effect of exogenous brain-derived neurotrophic factor （BDNF） on this pathway. Methods Four groups of one-month-old Sprague-Dawley rats received radiation doses of 0, 2, 10, and 20 Gy, respectively, in a single radiation. At three days after radiation, exogenous BDNF was injected stereotaxically into the bilateral hippocampus. Western blotting and RT-PCR were used to assess the levels of NFAT3/c4-related proteins in the hippocampus. Results The results of Western blotting and RT-PCR showed that the level of NFAT3/c4 was reduced in a dose-and time-dependent manner after ionizing radiation. Compared with the radiation alone group, the ionizing radiation plus BDNF group had significantly increased levels of NFAT3/c4 and CaN with increases in radiation dose and time. Conclusions Whole brain radiotherapy inhibits the CaN/NFAT3/c4 signaling pathway. Exogenous BDNF can promote the NFAT-dependent transcription and then improve the cognitive function.

关 键 词：NFAT3 C4 电离辐射 海马 大鼠 

分 类 号：R730.55[医药卫生—肿瘤]