一株产几丁质脱乙酰酶海洋细菌的筛选、鉴定及发酵优化  

Screening and Identification of a Marine Bacterium Producing Chitin Deacetylase and Optimization of Its Fermentation Condition

作　　者：来蒋丽 刘姝[1,2] 胡晟源 顾张慧 王淑军[2] 房耀维[1,2] 

机构地区：[1]淮海工学院海洋生命与水产学院,连云港222005 [2]江苏省海洋资源开发研究院,连云港222005

出　　处：《生物技术通报》2017年第11期153-159,共7页Biotechnology Bulletin

基　　金：江苏省自然科学基金面上项目(BK20151282);江苏省高校“青蓝工程”;江苏省“六大人才高峰”第十二批高层次人才项目(swyy-195);中国博士后科学基金(160034);青岛博士后基金;第48批“留学归国人员”科研启动基金;淮海工学院科研创新基金(Z2014016)

摘　　要：利用对硝基-N-乙酰苯胺筛选培养基从海州湾海域海泥中筛选获得产几丁质脱乙酰酶细菌MCDA02。经过形态学、生理生化和16S rDNA序列分析初步鉴定该菌株为解角质素微杆菌。通过单因素优化和正交试验优化,得出最优发酵条件。在单因素优化基础上,利用正交试验优化获得菌株MCDA02最优发酵条件为发酵温度25℃,培养基起始p H7.0,装液量50 m L/250 m L,几丁质3%,发酵时间48 h。在此发酵条件下,菌株MCDA02发酵水平达到158.47 U/m L,是优化前的3.2倍。试验结果为菌株MCDA02几丁质脱乙酰酶的进一步研究奠定基础。A strain MCDA02 producing chitin deacetylase was screened with the media added nitro-N-acetyl aniline from the marinemud of Haizhou bay. Then,it was identified as Microbacterium keratanolyticum through morphological,biochemical characteristics,and16 S r DNA sequence analysis. With single-factor strategy and orthogonal experiments,the fermentation condition was optimized as followings ：fermentation period of 48 h,initial p H of 7.0,culture temperature of 30℃,50 m L of liquid medium in a 250 m L flask,and 3% chitin. Underthe optimized conditions,the highest chitin deacetylase activity of strain MCDA02 reached 158.47 U/m L,which was about 3.2 times of thatbefore optimization. The results laid a foundation for further study of the chitin deacetylase from the strain MCDA02.

关 键 词：几丁质脱乙酰酶 海洋细菌 鉴定 产酶条件 

分 类 号：Q93[生物学—微生物学] TQ925[轻工技术与工程—发酵工程]