精氨酸加压素翻转脂多糖引起大鼠发热反应及其与棕色脂肪产热变化的关系  

Arginine Vasopressin Reverses fever Induced by Lipopolysaccharide in the Rats and Its Relationship with the Change in Brown Adipose Tissue Thermogenesis

作　　者：刘欣[1] 杨永录[2] 胥建辉[2] 刘畅 

机构地区：[1]兰州大学第一医院重症医学科,730000 [2]成都医学院体温与炎症四川省高校重点实验室,610500

出　　处：《医学研究杂志》2017年第11期76-80,共5页Journal of Medical Research

基　　金：四川省教育厅科研基金资助项目(15ZA0251)

摘　　要：目的研究外周给精氨酸加压素(AVP)对脂多糖(LPS)引起大鼠发热和棕色脂肪产热以及血清中IL-1β和PGE2水平的变化。方法实验用成年雄性SD大鼠,在23℃环境温度下,明暗时间各12h。用无线遥测系统连续测量大鼠体核温度(Tc)、棕色脂肪温度(T_(BAT))和活动。10:00时或11:30时分别给大鼠腹腔注射LPS(50μg/kg)、AVP(10μg/kg)或V1a受体阻断剂(30μg/kg)。用ELISA法测定血清IL-1β和PGE_2的含量。结果腹腔注射LPS后TBAT升高出现在Tc升高之前。腹腔V1a受体阻断剂能提高LPS引起发热反应。AVP能抑制LPS引起发热大鼠血清中IL-lβ和PGE2水平升高反应。结论 BAT产热在LPS引起的发热中有重要的作用。外周给AVP可通过抑制棕色脂肪产热和降低血液中IL-lβ和PGE2的浓度而翻转LPS发热反应。内源性AVP也有限制LPS发热的作用。Objective To investigate the effect of peripheral administration of arginine vasopressin （AVP） on lipopolysaccharide （LPS）-induced fever in the rats, and its relationship with the change in brown adipose tissue （BAT） thermogenesis and Interleukine-1β （IL-1β） and prostaglandin E2 （PGE2）.Methods The core temperature （Tc）,BAT temperature （TBAT） and activity were measured by telemetry in adult male Sprague-Dawley rats at an ambient temperature of 23℃ during a 12h light：12h dark photoperiod （lights on at 06：00 and lights off at 18：00）. The rats were dosed intraperitoneally with LPS （50μg/kg）, AVP （10μg/kg） or V1a vasopressin receptor antagonist （V1a antagonist, 30μg/kg） at 10：00 or 10：30. The concentrations of IL-1β and PGE2 in serum were tested by ELISA.Results （1） The increases in TBAT following LPS preceded the increases in Tc. （2） Peripheral administration of AVP reversed LPS-induced fever and decreased BAT thermogenesis. （3） V1a antagonist enhanced the fever produced by LPS. （4） AVP could significantly attenuate LPS-induced IL-1β and PGE2 production.Conclusion （1） BAT thermogenesis plays an important role in LPS-induced fever. （2） Peripheral administration of AVP reversed LPS-induced fever by reduction of BAT thermogenesis and inhibition of IL-1β and PGE2. （3） Endogenous AVP can attenuate fever induced by LPS.

关 键 词：脂多糖 棕色脂肪 精氨酸加压素 退热 

分 类 号：R364.6[医药卫生—病理学]