小反刍兽疫病毒N基因部分序列分子演化规律  

Molecular evolution of N gene part sequence in peste des petits ruminants virus

作　　者：王净[1] 王鹏[2] 王长顺 

机构地区：[1]河北北方学院动物科技学院,河北张家口075131 [2]河北北方学院农林科技学院,河北张家口075131

出　　处：《中国兽医学报》2017年第12期2304-2309,共6页Chinese Journal of Veterinary Science

基　　金：河北省科技计划资助项目[11230405D-1(2013)-4;14236602D-3(2014);14236602D-2(2015)];河北省畜牧兽医局科技遍地计划资助项目(2015-1-05)

摘　　要：为分析中国小反刍兽疫的流行特点,本研究采用生物信息学方法,从NCBI下载小反刍兽疫病毒(PPRV)N基因,以最大似然法建立分子进化树。结果显示,中国PPRV流行株分布于Ⅳ系,有2个分支,2007-2008年中国西藏流行株与2003年印度毒株遗传关系最近,2013-2015年中国西藏流行株与2012年巴基斯坦毒株遗传关系最近。中国小反刍兽疫主要发生于山羊,2015年新疆巴州毒株来自阿尔卑斯野山羊,与2013年以来的毒株在同一分支。China-BJ_2014与China-XJBZ_2015的遗传距离为0.011 7。中国两簇PPRV分支均与西部邻国毒株遗传关系最近,目前出现多宿主和变异性的流行特点,本研究为有效防控PPRV疫情提供基本的科学依据。In order to analyze the molecular evolution characterization of peste des petits ruminants virus in China,the molecular evolutionary tree was established with the PPRV N gene sequences from NCBI by maximum likelihood method. The results showed that PPRV strains were clustered in two branch within the lineage IV cluster in China. The epidemic strains in China Tibet during 2007-2008 had the highest similarity to India strains in 2003. The epidemic strains in China during 2013-2015 had the highest similarity to Pakistan strains in 2012. China _ibex-XJBZ_2015 and other PPRVs that emerged during 2013-2014 were clustered in one small branch. The genetic distance was 0. 011 7 between China-BJ_2014 and China-XJBZ_2015. Preliminary analysis indicated that two branch clusters of PPRV had the highest similarity to western neighbor in China,at present, the popular feature of PPRVs is multi-host and variability. This study provided a basis for effective prevention and Control PPR epidemic.

关 键 词：PPRV N基因 分子进化树 最大似然法 遗传距离 

分 类 号：S852.65[农业科学—基础兽医学]