紫外-ARTP复合诱变选育达巴万星前体高产菌株  

Breeding of Dalbavancin Precursor High-Yield Strain by UV-ARTP Composite Mutagenesis

作　　者：王小连 郄丽萍[1] 马婕[1] 代明伟[1] 邓佩佩[1] 

机构地区：[1]华北制药集团新药研究开发有限责任公司微生物药物国家工程研究中心河北省工业微生物代谢工程技术研究中心,河北石家庄050015

出　　处：《化学与生物工程》2017年第12期59-62,共4页Chemistry & Bioengineering

基　　金：河北省科技条件建设项目(中央引导地方科技创新专项)(169676404G)

摘　　要：采用紫外-常压室温等离子体(ARTP)技术对达巴万星前体产生菌野野村放线菌属(Nonomuriaspp.)菌株DW-3-19进行复合诱变,并进行链霉素抗性突变选育,获得了具有稳定遗传性的高产菌株,该菌株发酵单位比出发菌株提高了68.7%。表明,ARTP技术可有效应用于放线菌的诱变选育,紫外-ARTP复合诱变可提高达巴万星前体发酵单位,降低生产成本,为短时间内获得稳定遗传的高产菌株提供了可行的方法。Dalbavancin precursor-producing strain Nonomuriaspp.DW-3-19 was breeded by UV-atmospheric and room temperature plasma（ARTP）composite mutagenesis and streptomycin-resistant mutant screening.The results showed that a high-yield strain possessing stable genetic performance could be obtained using the composite mutagenesis,and the transformation efficiency was increased by 68.7% over the starting strain.The results also show that ARTP mutagenesis can be effectively used for the mutagenic breeding of Actinomycetes.UV-ARTP composite mutagenesis can improve the fermentation unit of dalbavancin precursor,reduce production cost,and obtain the high-yield strain with stable genetic performance in a short time.

关 键 词：达巴万星前体 紫外诱变 ARTP诱变 致死率 正突变率 

分 类 号：TQ465.92[化学工程—制药化工] TQ920.1[轻工技术与工程—发酵工程]