新疆维吾尔族与汉族溃疡性结肠炎患者肠黏膜组织炎性细胞因子及信号传导蛋白和转录激活物3表达差异研究  

Study on difference of inflammatory cytokines and STAT3 expressions in intestinal mucosal tissue between Uyghur and Han patients with ulcerative colitis in Xinjiang region

作　　者：杨涛[1] 周禾[1] 崔旻[1] 姚萍[1] 谭庆华 

机构地区：[1]新疆医科大学第一附属医院消化科,乌鲁木齐830000

出　　处：《重庆医学》2017年第35期4938-4942,共5页Chongqing medicine

基　　金：新疆维吾尔自治区自然科学基金资助项目(2014211C033)

摘　　要：目的探讨新疆维吾尔自治区维吾尔族溃疡性结肠炎(UC)患者肠黏膜组织中炎性细胞因子及信号转导蛋白和转录激活因子3(STAT3)表达与汉族UC患者的差异。方法选取2015年6月至2016年10月该院住院UC患者30例(维吾尔族15例,汉族15例)作为UC组,另选取同期健康检查者26例(维吾尔族13例,汉族13例)作为对照组,取受试者肠黏膜组织采用反转录PCR检测干扰素-γ(IFN-γ)、白细胞介素(IL)-22、IL-17A、IL-17F mRNA表达水平,并采用免疫组织化学法进行STAT3的检测。结果与维吾尔族对照组相比,维吾尔族UC组患者IFN-γ、IL-22、IL-17A、IL-17FmRNA表达水平都上调,差异均有统计学意义(P<0.05);与汉族对照组相比,汉族UC组患者上述炎性因子mRNA表达水平都上调,差异均有统计学意义(P<0.05);而汉族UC组与维吾尔族UC组患者上述炎性因子mRNA表达水平比较,差异均无统计学意义(P>0.05)。通过免疫组织化学法,STAT3在肠黏膜上皮细胞细胞质中着色,汉族、维吾尔族UC组阳性表达率均高于相应对照组,差异均有统计学意义(P<0.05);而维吾尔族UC组与汉族UC组相比,差异无统计学意义(P>0.05)。结论在新疆维吾尔自治区,维吾尔族和汉族UC患者肠黏膜IFN-γ、IL-22、IL-17A、IL-17F及STAT3表达明显增高,但上述因子在维吾尔族与汉族患者UC的发生、发展中可能无明显差异。Objective To explore the difference of inflammatory cytokines and STAT3 expressions in intestinal mucosal tissue between Uyghur and Han patients with ulcerative colitis （UC） in Xinjiang Uyghur Autonomous Region. Methods Thirty inpatients （15 Uyghur cases and 15 Han cases） with UC in this hospital from June 2015 to October 2016 were selected as the UC group. Contemporaneous 26 individuals （13 Uyghur cases and 13 Han cases ） undergoing physical examination served as the control group. The colonic mucosa tissue were taken for detecting the mRNA expression of IFN-γ, IL-22, IL-17A and IL-17F by reversal transcription-polymerase chain reaction （RT-PCR）. The expression of STAT3 was detected by using the immunohistochemical staining technique. Results Compared with the Uyghur control group, the mRNA levels of IFN-γ, IL-22, IL-17A and IL-17F in the Uyghur UC group were up-regulated,the differences were statistically significant （P〈0.05） ;compared with the Han healthy control group, the mRNA levels of above inflammatory cytokines in the Han UC group were up-regulated, the differences were statistically significant （P〈0.05） ;but the mRNA levels of above inflammatory cytokines had no statistical difference between the Uyghur UC group and Han UC group （P〉0.05）. The immunohistochemical method found that the STAT3 was found to be colored in the cytoplasm of intestinal mucosal epithelial cells. The positive expression rate of STAT3 in Han and Uyghur UC groups was higher than that in the corresponding control group, the differences were statistically significant （P〈0.05） ,however there was no statistically significant difference between Uyghur UC group and Han UC group, the difference was not statistically significant （P 〉0.05）. Conclusion In Xinjiang Uyghur Autonomous Region, the expressions of intestinal mucosal IFN-γ, IL-22, IL-17A, IL-17F and STAT3 in Uyghur and Han patients with UC are significantly increased,but the above cytokines may has no obvious difference in UC occurren

关 键 词：结肠炎 溃疡性 干扰素-γ 白细胞介素22 白细胞介素17A 白细胞介素17F 信号转导蛋白和转录激活因子3 

分 类 号：R574.62[医药卫生—消化系统]