运用SSR分子标记分析蛹虫草遗传多样性  

Genetic Diversity Analysis of Cordyceps militaris Using SSR Molecular Markers

作　　者：何华奇[1] 刘敏祥[1] 汪滢[2] 茅文俊[2] 鲍大鹏[2] 

机构地区：[1]安徽科技学院生命科学学院,安徽凤阳233100 [2]国家食用菌工程技术研究中心,农业部南方食用菌资源利用重点实验室,上海市农业遗传育种重点开放实验室,上海市农业科学院食用菌研究所,上海201403

出　　处：《园艺学报》2017年第11期2195-2202,共8页Acta Horticulturae Sinica

基　　金：安徽省2015年高等学校省级质量工程项目(2015ckjh027);上海市科委平台建设项目(16DZ2281300)

摘　　要：以蛹虫草全基因组为模板查找Simple Sequence Repeats(SSR)位点并设计筛选引物进行聚类分析。从100对SSR引物中选出30对多态性高、重复性好的SSR引物,共获得215条多态性条带。根据遗传相似系数(GS)采用不加权成对群算术平均法(UPGMA)进行遗传相似性聚类:材料间GS值的变化范围为0.527~0.995,以GS值为0.761水平可将46株蛹虫草分为6类;菌株亲缘关系与地域来源有一定相关性,且栽培菌株多为同一品种。The Cordyceps militaris genome was used as template to identify the SSR loci,to design the SSR primers,and the subsequent repeatable polymorphic bands were utilized for cluster analysis. The results showed that 30 SSR primers generated 215 highly repeatable polymorphic bands. Based on the estimation of genetic similarity coefficient（GS）calculated with unweighted pair group method arithmetic average（UPGMA）clustering method,the variation range of GS values were from 0.527 to 0.995,and 46 C. militaris strains could be divided into 6 groups with a cutoff of the GS value of 0.761. There was a certain correlation between the genetic relationship and the geographical origin of the strains. Interestingly,the strains used for commercial cultivation were genetically similar to each other.

关 键 词：蛹虫草 Simple SEQUENCE Repeats(SSR) 遗传相似系数(GS) 聚类分析 

分 类 号：S567[农业科学—中草药栽培] S646[农业科学—作物学]