石菖蒲挥发油微乳对慢性应激小鼠记忆的影响及其机理研究  

Shichangpu Volatile Oil Microemulsion Can Improve the Memory of Mice with Chronic Stress

作　　者：王光明[1] 李绍林[1] 蔡琳[1] 刘卫海[1] 

机构地区：[1]广东食品药品职业学院,广东广州510520

出　　处：《新中医》2017年第12期4-7,共4页New Chinese Medicine

基　　金：广东省科技计划项目(2013B021800173);广东省中医药局项目(20141211);广东食品药品职业学院院级项目(2013YZ006)

摘　　要：目的:建立慢性应激小鼠模型,观察石菖蒲挥发油微乳对慢性应激小鼠记忆的调节作用,并初步探讨其作用机制。方法:将36只昆明小鼠随机分为3组,每组12只,分别为空白对照组、慢性应激组、慢性应激+石菖蒲挥发油微乳组(给药组)。空白对照组正常喂养,慢性应激组每天进行8 h"慢性应激束缚实验",给药组给予石菖蒲挥发油微乳4.5 g/(kg·d)灌胃,同时与慢性应激组同步进行"慢性应激束缚实验"。观察小鼠一般状态并测量其体质量,利用Morris水迷宫观察小鼠的空间学习记忆能力,并检测小鼠血清糖皮质激素水平。结果:实验第28天时,与空白对照组比较,慢性应激组小鼠体质量明显下降,差异有统计学意义(P<0.05)。与慢性应激组比较,给药组小鼠的体质量增长明显,差异有统计学意义(P<0.05)。与空白对照组比较,慢性应激组小鼠在训练期间的平均潜伏期明显较长,平台穿越次数也显著减少,血浆皮质醇水平较高,差异均有统计学意义(P<0.01)。与慢性应激组比较,给药组小鼠的训练平均潜伏期缩短,平台穿越次数增多,血清中皮质醇水平明显下降,差异均有统计学意义(P<0.01)。结论:石菖蒲挥发油微乳具有改善慢性应激小鼠记忆的作用,其机制与降低血清糖皮质激素水平有关。Objective： To establish a chronic stress mouse model, and observe the regulatory effect of Shichangpu volatile oil microemulsion on the memory of mice model, so as to discuss its mechanism preliminarily. Methods： Divided 36 Kunming （KM） mice into 3 groups randomly, 12 cases in each group, namely blank control group, chronic stress group and combination of chronic stress and Shichangpu volatile oil microemulsion group （the treatment group）. Blank control group received the normal diet, chronic stress group received ＂the chronic restraint stress experiment＂, and the treatment group received the intragastric administration with 4.5 g/（kgod） of microemulsion of Shichangpu volatile oil as well as ＂the chronic restraint stress experiment＂. Observed the general state of mice, and measured their body mass. Observed the spatial learning and memory ability of mice by means of Morris water maze, and detected the level of serum glucocorticoid. Results： At the 28th day of the experiment, comparing with blank control group, body mass of the chronic stress group was decreased evidently, the difference being significant （P 〈0.05）. Comparing with chronic stress group, body mass of the treatment group was increased evidently, the difference being significant（P〈 0.05）. Comparing with blank control group, the latencies of mice in chronic stress group were evidently longer, times of passing through the platform were also reduced significantly, and levels of plasma cortisol were relatively high, differences being significant（P 〈0.01）. Comparing with the chronic stress group, the latencies of mice in the treatment group were shortened, times of passing through the platform were also increased, and levels of plasma cortisol were declined significantly, differences being significant （P 〈0.01）. Conclusion： The microemulsion of Shichangpu volatile oil has the function of improving the memory of mice with chronic stress, the mechanism of which is related to the decrease of levels of serum

关 键 词：石菖蒲 慢性应激 记忆 糖皮质激素 动物实验 小鼠 

分 类 号：R-332[医药卫生]