加热对肿瘤细胞微环境的影响及意义  

Influence and significance of heating on microenvironment of tumor cells

作　　者：王斌[1] 孙小单[2] 刘玉侠[3] 王首寒 程显斌 陈佳祺[1] 

机构地区：[1]吉林省肿瘤医院肝胆胰腺外科,长春130012 [2]吉林省肿瘤医院妇瘤二科,长春130012 [3]吉林省肿瘤防治研究所临床分子生物学研究室,长春1300120

出　　处：《癌症进展》2017年第10期1150-1152,1208,共4页Oncology Progress

摘　　要：目的探讨加热对体外培养的人肝癌细胞株SMMC-7721分泌的细胞因子的影响,以及细胞因子在肿瘤微环境中的作用,为热疗治疗肿瘤提供实验依据。方法体外培养人肝癌细胞株SMMC-7721至对数生长期,换成无血清培养基,将其放入恒温水浴箱中,42℃恒温加热2 h。将加热后的人肝癌细胞株SMMC-7721放回到37℃,5%CO2的培养箱中进行复温培养,取复温后不同时间(0、24、48、72 h)的人肝癌细胞株SMMC-7721培养上清进行TNF-α、IL-2、IFN-γ和VEGF等抗肿瘤细胞因子的检测,并与未加热的人肝癌细胞株SMMC-7721细胞培养上清进行比较。结果复温不同时间的人肝癌细胞株SMMC-7721培养上清中TNF-α、IL-2和IFN-γ抗肿瘤细胞因子的分泌浓度均较未加热的人肝癌细胞株SMMC-7721培养上清中上述肿瘤细胞因子的分泌浓度有不同程度的提高,而VEGF的分泌浓度逐渐下降。结论 42℃恒温加热2 h可以促进人肝癌细胞株SMMC-7721培养上清中IL-2、IFN-γ、TNF-α等抗肿瘤细胞因子分泌浓度的增加,减少促进肿瘤生长的细胞因子VEGF的分泌浓度。Objective To investigate the effect of heating on the cytokines secreted by liver cancer cells SMMC-7721, and to study the effect of these cytokines on cell microenvironment, to provide experimental evidence of hyperthermia for tumor treatment. Method Human liver cancer cell SMMC-7721 were cultured in vitro to the logarithmic growth phase, and then were replaced with a serum-free medium, placed in constant temperature water bath and heated for 2 hours at 42 ℃. After heating, the human liver cancer cell line SMMC-7721 were placed to cool down to 37℃, and were cultured again in an incubator with 5% CO2 by rewarming, the supernatant of the cultures at various time points after rewarming（0, 24, 48, 72 h） were taken to detect TNF-α, IL-2, IFN-γ, VEGF and other cytokines, and were compared with unheated SMMC-7721 cell culture supernatant. Result After rewarming for different duration, the secretion of cytokines TNF-α, IL-2, and IFN-γ in human cancer cells SMMC-7721 were generally increased as compared with unheated cultures, while VEGF secretion gradually decreased. Conclusion Constant heating at 42 ℃ for 2 h can promote the secretion of cytokines IL-2, IFN-γ, TNF-α, and other anti-tumor cytokines, while reduce the secretion of VEGF that can promote tumor growth.

关 键 词：热疗 肝癌细胞 细胞因子 肿瘤微环境 

分 类 号：R735.7[医药卫生—肿瘤]