miR-29c促进前列腺癌PC3细胞凋亡  

miR-29c promotes apoptosis in prostate cancer cell line PC3

作　　者：刘南京 李婷[1] 赵燕[1] 郝燕妮[1] 吴小候[2] 罗春丽[1] 

机构地区：[1]重庆医科大学检验医学院临床检验诊断学教育部重点实验室,重庆400016 [2]重庆医科大学附属第一医院泌尿外科,重庆400016

出　　处：《基础医学与临床》2017年第12期1706-1711,共6页Basic and Clinical Medicine

基　　金：国家自然科学基金(81072086)

摘　　要：目的研究miR-29c对前列腺癌PC3细胞凋亡的影响及其机制。方法以人正常前列腺上皮细胞系(RWPE-1)为对照,检测前列腺癌PC3细胞系中miR-29c、血管内皮生长因子(VEGF)及血管内皮生长因子受体2(VEGFR2)的表达;免疫荧光(IF)检测p-VEGFR2在细胞中的定位;miR-29c过表达腺病毒感染PC3,real-time PCR检测miR-29c及VEGF的表达;Western blot检测VEGF、t-VEGFR2、p-VEGFR2、BAX和Bcl-2的表达;hoechst 33258染色法检测PC3细胞凋亡;流式细胞计量术(FCM)检测PC3细胞早期凋亡率。结果与RWPE-1相比,PC3细胞miR-29c表达降低,VEGF及VEGFR2表达升高(P<0.001);与对照组相比,miR-29c过表达组VEGF、p-VEGFR2及Bcl-2的表达显著降低(P<0.001),BAX的表达显著升高(P<0.001),细胞凋亡与早期凋亡率显著增加(P<0.001)。结论重新表达miR-29c可以显著抑制VEGF/VEGFR2信号通路并促进PC3细胞凋亡。Objective To study the effect of miR-29 c on the apoptosis of prostate cancer cell line PC3. Methods The expression of miR-29 c,VEGF and VEGFR2 in RWPE-1 and PC3 were detected to verify the different between normal and cancer cell. The location of p-VEGFR2 was measured by immunofluorescence（ IF）. After PC3 cell were transfected by miR-29 c overexpression adenovirus, the expression level of miR-29 c, VEGF, t-VEGFR2,p-VEGFR2,BAX and Bcl-2 were measured by RT-PCR and Western blot,the cell apoptosis rate was detected by the kits of hoechst 33258 and FCM.Results The expression level of miR-29 c was lower and VEGF,VEGFR2 was higher in PC3 cell compared with that in RWPE-1（ P〈0. 001）. The expression level of miR-29 c and BAX were higher and VEGF,p-VEGFR2 and Bcl-2 were lower in Ad-miR-29 c group compared with that in control group（ P〈0. 001）.Higher apoptosis rate was detected in Ad-miR-29 c group compared with control group（ P〈0. 001）. Conclusions miR-29 c can promote apoptosis in prostate cancer cell PC3 by significantly inhibiting VEGF/VEGFR2 signaling.

关 键 词：前列腺癌 miR-29c 血管内皮生长因子 血管内皮生长因子受体 细胞凋亡 

分 类 号：R737.25[医药卫生—肿瘤]