HPV16E6与抑癌基因p53在口腔癌中的表达及临床意义  

Expression and correlation of HPV16E6 and tumor suppressor gene p53 in oral squamous cell carcinoma

作　　者：李怀奇[1] 武和明[1] 丁旭[1] 叶金海[1] 吴煜农[1] LI Huai-qi;WU He-ming;DING Xu;YE Jin-hai;WU Yu-nong(Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University,Department of Oral and Maxillofaeial Surgery, Affiliated Hospital of StomatoIogy, Nanjing Medical University. Nanjing 210029, Jiangsu Province, China)

机构地区：[1]南京医科大学口腔疾病研究江苏省重点实验室,南京医科大学附属口腔医院口腔颌面外科,江苏南京210029

出　　处：《上海口腔医学》2017年第6期615-618,共4页Shanghai Journal of Stomatology

基　　金：江苏高校优势学科建设工程资助项目(2014-37)

摘　　要：目的 :探讨HPV16E6基因和p53基因在口腔癌中的表达,以及口腔癌的病理分级和临床分期与HPV16E6基因及p53基因的相关性。方法:纳入173例口腔癌、98例口腔癌癌前病变以及79例癌周正常组织,采用免疫组织化学方法进行HPV16E6和p53表达水平检测,统计口腔癌、口腔癌癌前病变、癌周正常组织以及不同病理分级和临床分期的口腔癌中HPV16E6基因及p53基因的阳性表达情况,采用SPSS21.0软件包对数据进行χ2检验。结果:在口腔癌组织中,HPV16E6的阳性表达率为81.5%(141/173),p53的阳性表达率23.7%(41/173);在口腔癌癌前病变组织中,HPV16E6的阳性表达率为39.8%(39/98),p53的阳性表达率为57.1%(56/98);在口腔癌癌周正常组织中,HPV16E6的阳性表达率为2.5%(2/79),p53的阳性表达率为89.9%(71/79)。3组中HPV16E6的阳性表达率和p53的阳性率表达存在差异,其中,HPV16E6在口腔癌中的阳性表达率显著高于其他2组(P<0.05);而口腔癌中p53的阳性表达率显著低于其他2组。随着口腔癌患者临床分期和病理分级的增加,HPV16E6阳性表达率逐渐升高,而p53的阳性表达率则显著下降(P<0.05)。结论:HPV16E6蛋白和p53蛋白在口腔癌的发生与发展过程中可能发挥十分重要的作用。PURPOSE： To exploring the expression of PV16E6 gene and p53 gene in patients with oral carcinoma and the correlation between pathological grade and clinical stage of oral cancer and expression of HPV16E6 gene and p53 gene. METHODS： One hundred and seventy-three cases of oral cancer, 98 cases of oral precancerous lesions and 79 cases of peri-cancerous normal tissue were selected. The expression of HPV16E6 and p53 was detected by immunohistochemistry. The data were analyzed using SPSS 21.0 software package. RESULTS： In oral carcinoma, the expression rate of HPV16E6 was 81.5% （141/173）, the expression rate of p53 was 23.7% （41/173）; in oral precancerous lesions, the expression rate of HPV16E6 was 39.8% （39/98）, the expression rate of p53 was 57.1% （56/98）; in peri-cancerous normal tissues, the expression rate of HPV16E6 was 2.5%（2/79）, the positive expression rate of p53 was 89.9%（71/79）. There was significant difference in the positive expression rate of p53 and HPV16E6 among 3 groups. The expression of HPV16E6 in oral cancer was significantly higher than in the other two groups（P〈0.05）; the expression rate of p53 in oral cancer was significantly lower than the other two groups. In addition, with the advance of clinical stage and pathological grade, the positive expression rate of HPV16E6 increased gradually, while the positive expression rate of p53 was significantly decreased （P〈0.05）. CONCLUSIONS： HPV16E6 protein and p53 protein may play an important role in the occurrence and progress of oral cancer.

关 键 词：HPV16E6 P53 口腔癌 

分 类 号：R739.8[医药卫生—肿瘤]