Bacopa monnieri extracts prevent hydrogen peroxide- induced oxidative damage in a cellular model of neuroblastoma IMR32 cells  

作　　者：Gaurav Bhatia Vikram Dhuna Kshitija Dhuna Manpreet Kaur Jatinder Singh 

机构地区：[1]Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar-143005, Punjab, India [2]Department of Biotechnology, DAV College, Amritsar-143006, Punjab, India [3]Department of Human Genetics, Guru Nanak Dev University, Amritsar-143005, Punjab, India

出　　处：《Chinese Journal of Natural Medicines》2017年第11期834-846,共13页中国天然药物（英文版）

基　　金：supported by grants from the Department of Science and Technology (DST), Ministry of Science and Technology, New Delhi under order No. SR/FT/LS-163 and University with Potential for Excellence (UPE) Scheme, University Grants Commission, New Delhi

摘　　要：Neurodegenerative diseases are the consequences of imbalance between the production of oxidative stress and its nullification by cellular defense mechanisms. Hydrogen peroxide(H_2O_2), a precursor of deleterious reactive oxygen species, elicits oxidative stress, resulting in severe brain injuries. Bacopa monnieri is well known for its nerve relaxing and memory enhancing properties. The present study was designed to evaluate the protective effects of extracts from Bacopa monnieri against H_2O_2 induced oxidative stress using a cellular model, neuroblastoma IMR32 cell line. The protective potential of methanolic, ethanolic, and water extracts of B. monnieri(BM-MEx, BM-EEx, and BM-WEx) was evaluated using MTT assay. Although, all the B. monnieri extracts were found to protect cells against H_2O_2-mediated stress but BM-MEx showed significantly greater protection. UPLC analysis of BM-MEx revealed various polyphenols, including quercetin, catechin, umbelliferone, and caffeic acid predominance. Further, BM-MEx was found to possess considerable greater neuroprotective potential in comparison to the standard polyphenols such as quercetin, catechin, umbelliferone, and caffeic acid. The levels of antioxidant enzymes were significantly elevated after the pretreatment of BM-MEx and quercetin. The expression levels of oxidative stress markers, such as NF200, HSP70, and mortalin, were significantly alleviated after the pretreatment of BM-MEx as shown by immunofluorescence and RT-PCR. In conclusion, the present study demonstrated the protective effects of BM-MEx, suggesting that it could be a candidate for the development of neuropathological therapeutics.Neurodegenerative diseases are the consequences of imbalance between the production of oxidative stress and its nullification by cellular defense mechanisms. Hydrogen peroxide（H_2O_2）, a precursor of deleterious reactive oxygen species, elicits oxidative stress, resulting in severe brain injuries. Bacopa monnieri is well known for its nerve relaxing and memory enhancing properties. The present study was designed to evaluate the protective effects of extracts from Bacopa monnieri against H_2O_2 induced oxidative stress using a cellular model, neuroblastoma IMR32 cell line. The protective potential of methanolic, ethanolic, and water extracts of B. monnieri（BM-MEx, BM-EEx, and BM-WEx） was evaluated using MTT assay. Although, all the B. monnieri extracts were found to protect cells against H_2O_2-mediated stress but BM-MEx showed significantly greater protection. UPLC analysis of BM-MEx revealed various polyphenols, including quercetin, catechin, umbelliferone, and caffeic acid predominance. Further, BM-MEx was found to possess considerable greater neuroprotective potential in comparison to the standard polyphenols such as quercetin, catechin, umbelliferone, and caffeic acid. The levels of antioxidant enzymes were significantly elevated after the pretreatment of BM-MEx and quercetin. The expression levels of oxidative stress markers, such as NF200, HSP70, and mortalin, were significantly alleviated after the pretreatment of BM-MEx as shown by immunofluorescence and RT-PCR. In conclusion, the present study demonstrated the protective effects of BM-MEx, suggesting that it could be a candidate for the development of neuropathological therapeutics.

关 键 词：Antioxidant ENZYMES Bacopa monnieri H2O2 IMR32 NEUROBLASTOMA NF200 HSP70 Mortalin 

分 类 号：R965[医药卫生—药理学]