细胞质外功能σ因子在牙龈卟啉单胞菌生物膜形成中的作用  

Role of ECF sigma factor in biofilm formation of Porphyromonas gingivalis

作　　者：朱赛玲[1] 孙伟莲[2] 王利民[1] 

机构地区：[1]中国人民解放军第一一七医院口腔科,浙江省杭州市310000 [2]浙江大学第二附属医院口腔内科

出　　处：《江苏医药》2017年第19期1361-1363,共3页Jiangsu Medical Journal

摘　　要：目的研究细胞质外功能σ因子在牙龈卟啉单胞菌生物膜形成中的作用。方法将牙龈卟啉单胞菌培养物的浊度[600nm处吸光度(A_(600))值]调节至0.1,接种并厌氧培养2d后,取培养基加入0.1%结晶紫溶液0.5ml,溶解保留在生物膜中的结晶紫后测量A_(600)值。对怀疑与生物膜形成相关的细胞质外功能σ因子的菌株,再次培养后重复前述实验过程。结果所有5种破坏细胞质外功能σ因子基因的牙龈卟啉单胞菌在指数期的生长比普通菌株更慢(P<0.05);其中,PGN_1740突变体生长最为缓慢。PGN_0274和PGN_1740突变体的生物膜相对质量高于普通菌株(P<0.05)。结论牙龈卟啉单胞菌细胞质外功能σ因子参与生物膜形成;其中,PGN_0274和PGN_1740在生物膜形成中发挥关键作用。Objective To investigate the role of extracytoplasmic function（ECF） sigma factor in the biofilm formation of Porphyromonas gingivalis. Methods The turbidity of Porphyromonas gingivalis cultures was adjusted to absorbance at 600 nm（A600 ） of 0.1 and seeded into 12-well plates. After anaerobic incubation for two days, the medium was removed and 0.5 ml of 0.1% crystal violet solution was added. After dissolving the crystal violet retained in the biofilm,A600 was measured. The strain of ECF sigma factor suspected to be associated with biofilm formation was cultured repeatedly with the procedure mentioned above. Results The growth of all five Porphyromonas gingivalis strains disrupted the ECF sigma factor gene was slower than that of the common strains in the exponential phase（P〈0. 05） ,of which the growth of PGN 1740 mutant was the slowest. The relative biofilm qualities of PGN_0274 and PGN_1740 mutants were higher than those of common strains （P〈0. 05）. Conclusion The ECF sigma factor of Porphyroznonas gingivalis is involved in biofilm formation,in which PGN 0274 and PGN 1740 play a key role.

关 键 词：细胞质外功能σ因子 牙龈卟啉单胞菌 生物膜 

分 类 号：R781[医药卫生—口腔医学]