氯金酸修饰对再生电极检测性能的影响  

Influence of Chloroauric Acid Modification on Detection Performance of Regenerated Electrode

作　　者：刘永玲 李立[1,3] 李文娇 赵勇 卢瑛[1,2,4,5] 

机构地区：[1]上海海洋大学食品学院,上海201306 [2]上海水产品加工及贮藏工程技术研究中心 [3]农业部水产品贮藏保鲜质量安全风险评估实验室,上海201306 [4]食品热加工技术研究发展中心(亚洲),上海201306 [5]食品科学与工程国家级实验教学示范中心(上海海洋大学),上海201306 [6]国家淡水水产品加工技术研发分中心,上海201306

出　　处：《山东农业大学学报（自然科学版）》2017年第6期870-875,共6页Journal of Shandong Agricultural University：Natural Science Edition

基　　金：上海市农委科技兴农重点攻关项目(沪农科攻字(2016)第4-4号);上海市科委地方院校能力建设项目(15320502100);上海市科委工程中心能力提升项目(16DZ2280300);上海市教育委员会科研创新项目(14YZ120)

摘　　要：为了探讨氯金酸对再生电极检测性能的影响,本文首先对电极的再生条件及氯金酸修饰条件进行了优化,然后对氯金酸修饰前后再生电极的检测性能进行了分析和评价。结果表明,在优化的再生条件下(0.1 mol/L柠檬酸-柠檬酸钠、p H 2.4、再生30 min),再生电极的电流可恢复至其原始电流的近85%。在优化的氯金酸修饰条件(308.89μg、90 min)下,再生电极的电流可增加近60%。此外,再生电极经氯金酸修饰后,其对单增李斯特菌的检测限由3.08×103cfu/m L降低至2.1×102 cfu/m L,提高了14.6倍。稳定性也提高了近10%。本研究结果显示,一次性电极经优化再生处理和氯金酸信号增强处理后具有重复使用的能力。In order to explore the influences of chloroauric acid on detection performances of regenerated electrode, firstly,the conditions of regeneration and chloroauric acid modification for electrode were optimized. Then analyzed and evaluatedbefore and after the gold chloride acid modified electrode regeneration detection performance. The results showed that, underthe optimized regeneration conditions （0.1 mol/L citric acid-sodium citrate, pH 2.4, 30 min regeneration time）, the current ofregenerated electrode could recover to about 85% of its original current. Under the optimized conditions of chloroauric acidmodification （308.89 μg、90 min）, the current of regenerated electrode increased nearly 60%. In addition, the detection limitof regenerated electrode for Listeria monocytogenes was from initial 3.08 × 103cfu/mL reduced to 2.1 × 102cfu/mL,increasing 14.6 times after modification by chloroauric acid. The stability has also improved nearly 10%. The research resultsshowed that the disposable electrode has the ability to reuse after regeneration and modification by chloroauric acid.

关 键 词：电极 再生液 氯金酸修饰 单增李斯特菌 检测性能 

分 类 号：O646.5[理学—物理化学]