反义TIF3逆转镉转化BALB/c-3T3细胞的致癌性  

Antisense TIF3 reverses the oncogenic potential of CdCl_2-transformed BALB/c-3T3 cells

作　　者：雷毅雄[1] Pius Joseph 吴中亮[1] Tong-man Ong 

机构地区：[1]广州医学院化学致癌研究所,广州510182 [2]National Institute for Occupational Safety and Health

出　　处：《卫生研究》2002年第4期255-257,共3页Journal of Hygiene Research

摘　　要：为探讨反义TIF3能否逆转镉转化细胞的致癌性 ,用磷酸钙介导转染法和G418细胞筛选技术 ,建立CdCl2 转化BALB c 3T3细胞反义TIF3稳定表达系统 ,再用软琼脂检测和裸鼠致瘤试验对这些转基因细胞的致癌性逆转情况进行鉴定。结果显示CdCl2 转化BALB c 3T3细胞中反义TIF3表达可逆转这些细胞的转化表型 ,与非转染细胞和载体转染对照细胞比较 ,转染反义TIF3的镉转化细胞在软琼脂上所形成的锚非依赖性生长集落减少 2 5 %～ 70 %。转染反义TIF3基因的镉转化细胞可延迟裸鼠出现肿瘤的时间 ,而且这些肿瘤的大小显著变小 ,肿瘤重量平均下降 5 0 8%～ 5 5 1%。提示反义TIF3mRNA表达可逆转镉转化细胞的致癌性 ;TIF3 (GenBank Accession Number AF 271072) is identified as a novel cadmium- responsive proto-oncogene. In order to determine whether the antisense TIF3 reverses the oncogenic potential of Cd-transformed BALB/c-3T3 cells or not, a stable expression system of CdCl 2-transformed BALB/c-3T3 cells with the expression vector containing TIF3 cDNA in the antisense orientation using calcium phosphate and G418 selection protocols is firstly established. Then, the reversal of the oncogenic potential of these cells is tested by soft agar and nude mouse tumorigenicity assay. The results demonstrated that expression of the antisense TIF3 in the CdCl 2-transformed BALB/c-3T3 cells results in reversal of the transformed phenotype of the cells. This is evidenced by a 25%-70% decrease in the number of anchorage-independent colonies growing on soft agar and the significant reduced tumorigenic potential of cells in nude mice compared with the corresponding controls. In addition to a significant delay in the onset of appearance of tumors, a significant reduction in size and a 50.8%-55.1% decrease in weight of the tumors are also observed in the mice injected with the TIF3 antisense expressing cells compared with the corresponding controls. The results indicate that antisense TIF3 mRNA expression reverses its oncogenic potential of Cd-transformed BALB/c-3T3 cells and may have therapeutic potential to cancer induced by cadmium.

关 键 词：镉 反义TIF3 逆转 致癌性 

分 类 号：R730.231.3[医药卫生—肿瘤]