鸡骨草分级多糖的体外抗氧化活性  

Antioxidant effect of polysaccharides from Abrus Cantoniensis Hance in vitro

作　　者：秦建鲜 黄锁义 

机构地区：[1]右江民族医学院药学院,广西百色533000 [2]广西中医药大学药学院,南宁530001 [3]右江民族医学院右江流域特色民族药研究广西高校重点实验室,广西百色533000

出　　处：《中国临床药理学杂志》2017年第23期2411-2415,共5页The Chinese Journal of Clinical Pharmacology

基　　金：国家中医药管理局"十二五"中医药重点学科中药化学建设基金资助项目[国中医药人教发(2012)32号];广西自然科学基金资助项目(2013GXNSFAA019240);广西重点学科药物化学建设基金资助项目(桂教科研[2013]16号);广西高校科技创新能力提升工程建设基金资助项目[桂教科研(2015)5号]

摘　　要：目的研究鸡骨草分级多糖的体外抗氧化活性。方法抑制H_2O_2诱导红细胞氧化溶血能力体系:从小白鼠眼眶取血,置肝素离心管中,从血浆分出红细胞,实验分成3组:实验组、溶剂对照组、空白对照组,实验组加0.5%红细胞悬浮液0.5 m L+不同浓度的鸡骨草多糖溶液0.2 m L,溶剂对照组加0.9%Na Cl0.5 m L+不同浓度的鸡骨草多糖溶液0.2 m L,空白组加0.9%Na Cl 0.5 m L+双蒸水0.2 m L。以Fe^(2+)-Vc诱导的小鼠肝匀浆脂质过氧化体系:小白鼠脱颈椎处死,迅速摘取肝组织,实验分成3组:实验组、对照组、空白组。实验组加肝组织匀浆1 m L+不同浓度的鸡骨草多糖1 m L,对照组加0.9%NaCl 1 m L+不同浓度的鸡骨草多糖1 m L,空白组加0.9%Na Cl 1 m L+双蒸水1 m L。用1,1-二苯基-2-1-二苯基-2-三硝基苯肼(DPPH)法清除DPPH自由基;用邻苯三酚自氧化法清除O2-自由基;以2,2'-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS)法测定总抗氧化能力体系;用铁离子螯合能力法测金属离子螯合能力;用铁离子还原力法测还原力能力。结果鸡骨草不同分子量多糖(PACL1、PACL2、PACL3、PACL)对DPPH自由基的清除率分别是95.57%,95.61%,92.80%,91.57%;其对O2-自由基的清除率分别是54.00%,46.45%,33.11%,28.82%;其对ABTS自由基的清除率分别是95.69%,62.53%,55.83%,55.92%;其对Fe2+的螯合能力分别是81.79%,81.29%,34.41%,35.38%;对Fe^(3+)的还原能力分别是50.38%,32.48%,41.21%,79.10%;其清除H2O2的能力分别是94.78%,61.50%,61.66%,98.86%;其清除NO的能力分别是68.06%,36.48%,57.19%,54.23%;其对H_2O_2诱导红细胞氧化溶血的影响为PACL1>PACL2>PACL3>PACL;其对Fe^(2+)-VC诱导小鼠肝匀浆脂质过氧化的影响为PACL2>PACL1>PACL3>PACL。结论鸡骨草多糖PACL、PACL1、PACL2、PACL3这4个组分均具有较强的抗氧化活性,其中分子量小的PACL1的抗氧化活性最强,且抗氧化活性与其自身的分子量呈负相关。Objective To study the antioxidant activity of polysaccharide from Abrus Cantoniensis Hance. Methods The system of inhibiting H2O2 induced hemolysis of erythrocytes： blood was taken from the orbit of the mouse eye,and then was placed into the heparin centrifuge tubeto separate the red blood cells from the plasma. The experiment was divided into three groups： The experimental group [0. 5% red blood cell suspension 0. 5 m L ＋ different concentrations of Jigucao polysaccharide solution 0. 2 m L（ JPS） ],solvent control group（ 0. 9% Na Cl 0. 5 m L ＋different concentrations of JPS 0. 2 m L）,blank control group（ 0. 9%Na Cl 0. 5 m L ＋ double distilled water 0. 2 m L）. The Fe^（2＋）-VCinducedmouse liver homogenate lipid peroxidation system： mice were sacrificed by cervical dislocation,rapid removal of liver tissue. The experiment wasdivided into three groups： experimental group with liver homogenate 1 m L ＋ different concentrations of JPS 1 m L,the control group with 0. 9% NaCl 1 m L ＋ different concentrations of JPS 1 m L,blank group with 0. 9% Na Cl 1 m L ＋ double distilled water 1 m L. The free radical scavenging was determined by 1,1-diphenyl-2-picylhydrazyl（ DPPH）. The O2-free radical scavenging was assayed by pyrogallol oxidation. Total antioxidant capacity system was measured by 2,2＇-azinobis-（ 3-ethylbenzthiazoline-6-sulphonate）. The chelating ability of metal ions was measured by iron ion. The iron ion reduction force method was used to measure the force capacity. H2O2 was eliminated by reference to literature. NO was removed by nitroprusside. Results DPPH free radical scavenging rate of Abrus Cantoniensis Hance with different molecular weight polysaccharides（ PACL1,PACL2,PACL3,PACL）were 95. 57%,95. 61%,92. 80%,91. 57%; O2-free radical scavenging rate of the four component were 54%,46. 45%,33. 11%,28. 82%. ABTS free radical scavenging rate of the four component respectively were 95. 69%,62. 53%. 55. 83%,55. 92%. Chelating ability of Fe2 ＋of the four component we

关 键 词：鸡骨草 多糖 与分子量呈负相关 抗氧化活性 

分 类 号：R28[医药卫生—中药学]