结肠癌中MEG3和GAS5的表达及与预后的关系  

Expression and clinical significance of MEG3 and GAS5 in the colorectal cancer

作　　者：司君利[1] 孟菲菲[2] 崔京远[3] 亓玉琴[2] 吴健婷 

机构地区：[1]青岛大学附属青岛市市立医院急诊科,山东青岛266011 [2]青岛大学附属青岛市市立医院消化科,山东青岛266011 [3]青岛大学附属青岛市市立医院普外科,山东青岛266011

出　　处：《中国现代普通外科进展》2017年第9期673-676,共4页Chinese Journal of Current Advances in General Surgery

摘　　要：目的:检测长链非编码RNA(lncRNA)母系表达基因3(MEG3)、生长特异抑制物5(GAS5)在结肠癌组织中的表达水平,分析GAS5与细胞周期蛋白P21、E2F1的相关性以探讨其作用机制。方法:收集2014年12月--2015年12月结肠癌患者手术切除标本(包括癌组织及对应的远端正常组织)。qRT-PCR检测63例结肠癌中MEG3、GAS5的表达,并分析其与临床病理参数的关系。Western blot法检测结肠癌中P21蛋白、E2F1蛋白的表达水平,并分析其与GAS5的相关性。结果:(1)结肠癌组织中MEG3的表达水平(7.76±1.38)显著低于正常组织(9.52±1.31),差异有统计学意义(P<0.05)。(2)结肠癌组织中GAS5的表达水平(3.98±1.15)显著低于正常组织(6.21±1.33),差异有统计学意义(P<0.05)。(3)在结肠癌组织(r=-0.627,P=0.000)及远端正常组织(r=-0.807,P=0.000)中,P21与E2F1的表达呈负相关。GAS5的相对表达水平与P21存在正相关(r=0.410,P=0.001),与E2F1存在负相关(r=-0.366,P=0.003)。(4)MEG3、GAS5高表达者较低表达者中位生存期明显延长(P<0.05)。结论:MEG3、GAS5在结肠癌中表达降低,表明其扮演抑癌基因的角色,P21和E2F1是GAS5发挥抑癌作用的重要靶点,检测MEG3、GAS5的表达水平对结肠癌预后有潜在价值。Objective： To detect the expression of long non-coding RNA （Long non-coding RNA, IncRNA） maternally expressed gene 3 （maternally expressed gene 3, MEG3）, specific growthinhibitor 5 （Growth arrest-specific 5, GAS5） and to analyze the correlation between GAS5 and cyclin P21,E2F1,so as to investigate the mechanism of GAS＆ Methods： From December 2014 to De- cember 2015,63 consecutive Patients with colorectal cancer admitted to Qingdao Municipal Hospital for surgical treatment were included into our study. We collect coiorectal cancer tissues and paired normal tissues. We tested the relative expression of MEG3, GAS5 genes by real-time quantitative PCR （RT-PCR）, and Western blot was used to detect the expression of P21 and E2F1 in colorectal cancer and to evaluate their correlations with GAS5. Results： The expression of MEG3 in cancer （7.76 ± 1.38） was lower than that in normal tissues（9.52 ± 1.31）P〈0.052.The expression of GAS5 was decreased in cancer tissues （3.98± 1.15） relative to the normal （6.21 ±1.33）P〈0.05.3. It showed that there were positive relationships between P21 and GAS5（r=0.410, P=0.001）,and there were negative relationships between E2F1 and GAS5（r=-0.366, P=0.003）. Conclusions： MEG3 and GAS5 were decreased in colorectal cancer, suggesting that they play inhibiting effects on the cancer. P21 and E2F1 are important target spots that GAS5 give play to the role of tumor suppressor.

关 键 词：结肠癌 长链非编码RNA 母系表达基因3 生长特异抑制物5 预后 

分 类 号：R735.35[医药卫生—肿瘤]