脉抒降脂及改善急性酒精性肝损伤的作用  

Effects of Maishu on Lipid-lowering and Improving Ethanol Induced Acute Liver Injury

作　　者：方欢乐[1] 黄永刚 杨保德 许毛 胡浩[3] 赵铭[3] 

机构地区：[1]西安培华学院医学院,西安710125 [2]青海睿元药物研究所有限责任公司,西宁810003 [3]西安交通大学医学部,西安710061

出　　处：《世界中医药》2017年第11期2744-2748,共5页World Chinese Medicine

基　　金：国家自然科学基金青年基金(81402924);青海省科技厅基础研究项目(J2015012)

摘　　要：目的:研究脉抒降脂及对急性酒精性肝损伤大鼠的保护作用。方法:采用高脂饲料喂养建立高脂动物模型,将雄性SD大鼠随机分为6组,即对照组、模型组、阳性给药组、脉抒高、中、低剂量组;除对照组外其余各组大鼠高脂喂养同时给药,4周后测定血清总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)及丙二醛(MDA)、超氧化物歧化酶(SOD)水平。另取大鼠60只,分为对照组、模型组、硫普罗宁组及脉抒高、中、低剂量组,用白酒灌胃诱导、建立大鼠酒精性脂肪肝模型,各组动物连续灌胃给药28 d,测定血清中TG、天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)水平,ELISA法测定血清极低密度脂蛋白(VLDL)水平;进行肝组织病理学检查。结果:和模型比较,脉抒高、中、低剂量组均对高脂大鼠体重未见明显影响;对高脂大鼠血清TC和HDL-C水平无明显影响;但可显著性降低高脂大鼠血清TG和LDL-C水平。同时能够升高大鼠血液中SOD水平,降低MDA水平(P<0.05,P<0.01)。可明显降低急性酒精性肝损伤大鼠血清ALT、AST、TG和VLDL水平,脉抒大剂量可改善急性酒精性肝损伤大鼠肝脏脂肪变性程度(P<0.05,P<0.01)。结论:脉抒对高脂大鼠有明显的辅助降血脂作用同时对急性酒精性肝损伤大鼠有明显的辅助保护作用。Objective： To investigate the protective effects of Maishu on acute liver injury and lipid-lowering. Methods： Hyperlipidemia model was induced by high-fat diet. Male SD rats were randomly divided into control group,model group,positive treatment group,Maishu high dose group,middle dose group and low dose group; Except control group,other groups were feeded with highfat diet and drugs. At the end of 4 weeks,the levels of serum TC,TG,LDL-C,HDL-C,SOD and MDA were determined. Another 60 rats were randomly divided into the control group,model group,tiopronin group,Maishu high dose group,middle dose group and low dose group. The model of alcoholic liver damage in mice was induced by intragastic administration of alcohol. Each group was given continuously by gavage 28 days. The serum levels of ALT,AST and TG were determined. The content of VLDL was determined by ELISA. Histopathological changes of the liver were observed by HE staining. Results： Comparing with the model group,Maishu high dose,middle dose and low dose had little or even no influence on mice＇s weight,and had no adverse effects on the levels of TC and HDL-C of hyperlipidemic rats. But it can significantly decrease the levels of TG ang LDL-C,as well as increase SOD and reduce the MDA content（ P 0. 05,P 0. 01）. It could significantly reduce ALT,AST,TG and VLDL on acute alcoholic liver injure in rats. Mashu high dose can improve hepatocyte-steatosis（ P 0. 05,P 0. 01）. Conclusion： Maishu is effective on blood-lipid regulation of the hyperlipidemia model rats and it has protective effects on ethanol-induced acute hepatic injury in mice.

关 键 词：脉抒 高血脂 抗氧化 酒精性肝损伤 

分 类 号：R284[医药卫生—中药学]