亚砷酸钠致小鼠乳腺癌作用机制研究  

Study on the mechanism of sodium arsenite induced breast cancer in mice

作　　者：董雪[1] 曹阳[1] 李婷[1] 苗晨曦 阎琪[1] DONG Xue;CAO Yang;LI Ting;MIAO Chenxi;YAN Qi(Changchun University of Chinese Medicine, Changchun 130117, China)

机构地区：[1]长春中医药大学,长春130117

出　　处：《长春中医药大学学报》2017年第6期880-882,共3页Journal of Changchun University of Chinese Medicine

基　　金：吉林省教育厅项目(20163)

摘　　要：目的研究亚砷酸钠(Na As O2)对小鼠乳腺上皮细胞(NMu MG)的影响,为砷致乳腺癌机制提供理论依据。方法以小鼠乳腺上皮细胞为模型,研究亚砷酸钠对乳腺上皮细胞形态的影响;经流式细胞仪检测亚砷酸钠对小鼠乳腺上皮细胞凋亡的影响;实时定量RT-PCR检测细胞周期基因P21 m RNAs表达水平。结果 Na As O2对NMu MG具有毒性效应,在一定浓度范围内,随Na As O2浓度升高,细胞形态明显改变,凋亡明显增多,且呈剂量-效应关系;细胞增殖的抑制基因P21 m RNA水平增高了18.12倍。结论亚砷酸钠可能通过上调P21基因的表达来抑制NMu MG增殖,可能是砷中毒引起乳腺癌的发生机制之一。Objective To study the effect of sodium arsenite（NaAsO2） on mouse mammary epithelial cells（NMuMG）, and to provide a theoretical basis for arsenic induced breast cancer mechanism. Methods The effect of sodium arsenite on the morphology of mammary epithelial cells was studied by using mouse mammary epithelial cells as models. The effect of sodium arsenite on the apoptosis of mouse mammary epithelial cells was detected by flow cytometry. Cell cycle gene P21 mRNAs expression level was detected by real-time quantitative RT-PCR. Results NaAsO2 has toxic effect on NMuMG. In a certain range of concentration, with the increase of NaAsO2 concentration,the cell morphology changes obviously, and the apoptosis increases obviously, and dose-response relationship exists.The level of P21 mRNA, an inhibitor of cell proliferation, was increased by 18.12 times. Conclusion Sodium arsenite may inhibit the proliferation of NMuMG by up-regulating the expression of P21 gene, which may be one of the mechanisms of arsenic poisoning caused by breast cancer.

关 键 词：亚砷酸钠 增殖 P21基因 乳腺癌 

分 类 号：R914.5[医药卫生—药物化学]