DNA损伤同源重组修复相关蛋白在软组织平滑肌肉瘤中的表达及其预后意义  

Expression of DNA Damage Homologous Recombination Repair Related-Proteins in Soft Tissue Leiomyosarcoma and Its Clinical Significance

作　　者：郝梦泽 李锋 邢培培 李婷 杨吉龙 

机构地区：[1]天津医科大学肿瘤医院,国家肿瘤临床医学研究中心,天津市肿瘤防治重点实验室,天津市恶性肿瘤临床医学研究中心,天津300060

出　　处：《肿瘤学杂志》2017年第11期937-944,共8页Journal of Chinese Oncology

基　　金：国家自然科学基金(81372872)

摘　　要：[目的]探讨DNA损伤同源重组修复相关蛋白在软组织平滑肌肉瘤(leiomyosarcoma,LMS)中的表达及其预后意义。[方法 ]采用免疫组化SP法检测61例LMS中RAD51重组酶(RAD51)、RAD52同源性DNA修复蛋白(RAD52)、ATM丝氨酸/苏氨酸(ATM)及ATR丝氨酸/苏氨酸(ATR)的表达。[结果]61例LMS患者的1年、5年和10年生存率分别为87%、46%和19%;中位生存期(overall survival,OS)为44.7个月,中位无病生存期(disease-free survival,DFS)为30.3个月,中位无进展生存期(progress-free survival,PFS)为17.0个月。LMS中RAD51、RAD52、ATM及ATR的阳性表达率分别为45.9%(28/61)、62.3%(38/61)、16.4%(10/61)和60.7%(37/61)。Kaplan-Meier分析显示RAD51,RAD52,ATM和ATR是影响LMS患者OS的重要因素(P<0.05)。Cox回归分析显示RAD52、ATM和ATR是影响LMS患者OS的独立预后因子(P<0.05)。此外,ATM是LMS患者DFS的独立影响因子(P<0.05),而RAD52是PFS的独立影响因子(P<0.05)。[结论]DNA损伤同源重组修复相关蛋白RAD52、ATM和ATR可作为评估LMS预后的独立因子。[Objective] To explore the correlation of the expression of DNA damage homologous recombination repair related-proteins in soft tissue leiomyosarcoma （LMS）,as well as its relationship with patients clinical prognosis. [Methods] 61 cases of LMS formalin-fixed paraffin-embedded tissue microarray to detect the expression of LMS in RAD51 recombinase （RAD51）,RAD52 homolog,DNA repair protein （RAD52）,ATM serine/threonine （ATM） and ATR serine/threonine （ATR） were using immunohistochemistry SP method. [Results] The 1-year,5-year and 10-year survival rates of 61 soft tissue LMS patients were 87%,46% and 19%,respectively. Moreover,the median OS time was 44.7 months,median DFS time was 30.3 months and median PFS time was 17.0 months. The positive expression rates of RAD51,RAD52,ATM and ATR in LMS were 45.9%（28/61）,62.3%（38/61）,16.4%（10/61） and 60.7%（37/61）,respectively. Kaplan-Meier analyses indicated that RAD51,RAD52,ATM and ATR were all important prognosticators on OS of LMS patients（P〈0.05）. Furthermore,Cox regression method showed that RAD52,ATM and ATR were independent prognostic factors in LMS patients with OS（P〈0.05）. Moreover,ATM was the independent prognostic predictors of LMS patients’ DFS（P〈0.05） and RAD52 with the PFS（P〈0.05）.[Conclusion] DNA damage homologous recombination repair proteins RAD52,ATR and ATM could act as the independent prognostic factors for soft tissue LMS patients.

关 键 词：软组织平滑肌肉瘤 RAD51重组酶 RAD52同源性DNA修复蛋白 ATM丝氨酸/苏氨酸 ATR丝氨酸/苏氨酸 

分 类 号：R738.6[医药卫生—肿瘤]