miR-130a-3p通过抑制CXCL12抑制鼻咽癌细胞增殖与侵袭  

The effect of miR-130a-3p on function of human nasopharyngeal carcinoma cells CNE-1 by down-regulating the expression of CXCL12

作　　者：刘春丽[1] 张洁[1] 齐志伟[1] 梁媛[1] 黄跃雁[1] 张淑君[1] 

机构地区：[1]承德医学院附属医院耳鼻喉科,河北承德067000

出　　处：《中国实验诊断学》2017年第12期2168-2170,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨miR-130a-3p对人鼻咽癌细胞CNE-1功能的影响及其可能的靶向基因。方法采用脂质体介导方法将miR-130a-3p模拟物(实验组)或对照模拟物(对照组)转染CNE-1细胞,分别用CCK-8法和Transwell试验检测细胞增殖侵袭和迁移能力变化,Western Blot检测趋化因子CXCL12蛋白表达。结果相较对照组,实验组CNE-1细胞的增殖,侵袭和迁移能力增强(P<0.05),细胞中CXCL12蛋白表达水平明显下调(P<0.05)。结论 miR-130a-3p可能通过调节CXCL12蛋白表达抑制人鼻咽癌细胞CNE-1细胞的增殖、侵袭和迁移。Objective to investigate the effect of miR-130a-3p on the function of human nasopharyngeal carcinoma cell CNE-1 and its possible target genes.Methods using liposome mediated method,analogue of miR-130a-3p （experi-mental group）or analogue control （control group）were transfected into CNE-1 cells,respectively using CCK-8 method and Transwell test for cell proliferation migration and invasion changes,Western Blot detection of chemokine CXCL12 protein expression.Results compared with the control group,the proliferation,invasion and migration ability of CNE-1 cells in the experimental group were increased （P 〈0.05）,and the expression level of CXCL12 protein in the cells was significantly lower（P 〈0.05）.Conclusion miR-130a-3p may inhibit the proliferation,invasion and migration of human nasopharyngeal carcinoma cell line CNE-1 by regulating the expression of CXCL12 protein.

关 键 词：鼻咽癌 趋化因子 CXCL12 miR-130a-3p CNE-1细胞 

分 类 号：R739.63[医药卫生—肿瘤]