HPLC同时测定鸡血藤中芒柄花素和美迪紫檀素等6个黄酮类成分  

Simultaneous determination of formononetin,medicarpin and other 4 flavonoidsin Spatholobi Caulisby HPLC

作　　者：杨冉冉 索亚然 乔艺涵 罗隽[1,2] 冯娅茹 赵崇军 马志强[1,2] 林瑞超 邹迪新[3] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]中药品质评价北京市重点实验室,北京100102 [3]内蒙古医科大学药学院,内蒙古010110

出　　处：《药物分析杂志》2017年第12期2139-2144,共6页Chinese Journal of Pharmaceutical Analysis

基　　金：2015年度中医药行业科研专项-中药饮片质量保障系统研究(一)(201507002)

摘　　要：目的:建立HPLC同时测定鸡血藤中原儿茶酸、儿茶素、表儿茶素、甘草素、芒柄花素、美迪紫檀素含量的方法,为鸡血藤药材质量标准改进提供参考。方法:采用ZORBAX SB-C18色谱柱(4.6 mm×150 mm,5μm),以乙腈(A)-0.2%磷酸(B)为流动相进行梯度洗脱,流速1 m L·min^(-1),检测波长为260 nm(检测原儿茶酸、芒柄花素)和280 nm(检测儿茶素、表儿茶素、甘草素、美迪紫檀素),柱温30℃。结果:原儿茶酸、儿茶素、表儿茶素、甘草素、芒柄花素、美迪紫檀素的质量浓度分别在2.286~228.6μg·m L^(-1)(r=0.999 9)、2.174~217.4μg·m L^(-1)(r=0.999 9)、2.79~279μg·m L^(-1)(r=0.999 9)、0.424~42.4μg·m L^(-1)(r=0.999 9)、2.426~242.6μg·m L^(-1)(r=0.999 9)、0.339~33.92μg·m L^(-1)(r=0.999 8)的范围内线性关系良好,加样回收率分别为99.8%(RSD=1.0%)、99.8%(RSD=2.5%)、101.2%(RSD=2.3%)、100.8%(RSD=1.8%)、100.9%(RSD=2.3%)、100.6%(RSD=2.4%)。11批药材中原儿茶酸、儿茶素、表儿茶素、甘草素、芒柄花素、美迪紫檀素含量范围分别为0.250~0.898、1.447~5.662、1.759~11.925、0.035~0.134、0.218~0.535、0.065~0.585 mg·g^(-1)。结论:该方法可用于同时测定鸡血藤中6个黄酮类成分的含量,为该药材的质量标准改进提供参考。Objective： To establish an HPLC method for simultaneous determination of protocatechuic acid, catechol, L-epicatechin, liquiritigenin, formononetin and medicarpinin Spatholobi Caulis, and to offer reference for improvement of the standard of the drug. Methods： Separation was performed on a ZORBAX SB-Cls column （ 4.6 mm × 150 mm5 μm ） and the mobile phase was acetonitrile-0.2% phosphoric acid with gradient elution. The flow rate was 1 mL·min-1 and the wavelength was set at 260 nm for protocatechuic acid and formononetin and at 280 nm for catechol, L-epicatechin, liquiritigenin and medicarpin. The column temperature was 30 % . Results： The linear ranges of protocatechuic acid, catechol, L-epicatechin, liquiritigenin, formononetin and medicarpin were 2.286-228.6 μg·mL-1 （ r=0.999 9 ）, 2.174-217.4μg·mL-1 （ r=0.999 9 ）, 2.79-279 μg·mL-1（ r=0.999 9 ）, 0.424-42.4 μg·mL-1 （ r=0.999 9 ）, 2.426-242.6 μg·mL-1（ r=0.999 9 ） and 0.339-33.92μg·mL-1（ r=0.999 8 ）, respectively. The average recoveries （ n=6 ） were 99.8% （ RSD=1.0% ）, 99.8% （ RSD=2.5% ）, 101.2% （ RSD=2.3% ）, 100.8% （ RSD=1.8% ）, 100.9% （ RSD=2.3% ） and 100.6% （ RSD=2.4% ）, respectively. The content ranges of protocatechuic acid, catechol, L-epicatechin, liquiritigenin, formononetin and medicarpinin Spatholobi Caulis were 0.250-0.898 μg·mL-1, 1.447-5.662μg·mL-1, 1.759-11.925 μg·mL-1, 0.035-0.134μg·mL-1, 0.218-0.535μg·mL-1 and 0.065-0.585 μg·mL-1, respectively. Conclusion： The developed method is suitable for simultaneous determination of 6 components in Spatholobi Caulis, thus providing reference for the improvement of the standard of the drug.

关 键 词：鸡血藤 原儿茶酸 儿茶素 表儿茶素 甘草素 芒柄花素 美迪紫檀素 高效液相色谱 

分 类 号：R917[医药卫生—药物分析学]