在激光的作用下卟啉类光敏剂Photosan对人喉癌细胞中STAT3信号传导通路的相关调控蛋白的影响  

Under the action of laser class porphyrin photosensitizer Photosan of STAT3 signaling pathway in human laryngeal cancer cells related to the impact of regulatory proteins

作　　者：郭慧[1] 韩锦华[1] 李东波[1] 李晓燕[1] 

机构地区：[1]大连大学附属新华医院耳鼻喉科,辽宁大连116021

出　　处：《中国医学文摘（耳鼻咽喉科学）》2017年第6期296-298,302,共4页Chinese Medical Digest(Otorhinolaryngology)

基　　金：大连市卫生局课题(1311084)

摘　　要：目的利用激光作用于喉癌细胞,探讨药物光敏剂Photosan对人喉癌Hep-2细胞系增殖和凋亡的影响,及STAT3传导通路调控蛋白P-STAT3、Cyclin Dl、Bcl-2的表达影响。方法体外培养人喉鳞状细胞癌Hep-2细胞株,光动力作用于喉癌细胞,实验分A组(光敏剂处理实验组)及对照组,对照组分为B组(不加光敏剂的单纯照光组)、C组(只加光敏剂不照光组)和D组(既不加光敏剂又不照光的空白组)。流式细胞技术检测不同试验组的细胞凋亡情况、不同试验组P-STAT3及其靶基因产物Cyclin Dl、Bcl-2蛋白表达水平。结果在激光的作用下光敏剂Photosan抑制喉癌细胞增殖,促进细胞凋亡,STAT3信号传导通路蛋白P-STAT3、Cyclin Dl、Bcl-2表达受到明显的抑制。结论受激发后的光敏剂Photosan能够降低STAT3相关调控蛋白的表达,抑制喉癌细胞增殖,促进其凋亡。Objective Using photodynamic action in laryngeal cancer cells,discuss the Hep-2 to the influence of cell proliferation and apoptosis,and the STAT3 pathway regulating protein the expression of P-STAT3 and Cyclin Dl,Bcl-2. Methods In vitro Hep-2 laryngeal squamous cell carcinoma cell lines,photodynamic action in laryngeal cancer cells,the experiment is divided into,group A（photosensitizer treatment group）,group B（without photosensitizer pure light control group）,group C（add photosensitizer not only the light of photosensitizer control group）,and group D（without photosensitizer and did not as the light of the blank control group）. Flow cytometry technology to detect different experimental cell apoptosis,and different group of P-STAT3 and its target gene product Cyclin Dl,Bcl-2 protein expression level. Results Under the action of laser,photosensitizer Photosan inhibition of laryngeal cancer cell proliferation,promote apoptosis,STAT3 signaling pathways protein P-STAT3,Cyclin Dl,Bcl-2 expression was obvious inhibition. Conclusion After stimulated photosensitizer Photosan could reduce the expression of STAT3 related regulatory proteins,inhibition of laryngeal cancer cell proliferation,promote apoptosis.

关 键 词：光动力治疗 喉癌 增殖 凋亡 

分 类 号：R734.1[医药卫生—肿瘤]