青藤碱通过TNF-α、IL-23及β-catenin抑制人结肠癌细胞增殖  

Inhibition of proliferation of human colon cancer cells with sinomenine via TNF-α,IL-23 and β-catenin

作　　者：周龙翔[1] 张曙[1] 饶雷平[1] 

机构地区：[1]上海市第六人民医院金山分院普通外科,上海201599

出　　处：《西南国防医药》2017年第12期1256-1259,共4页Medical Journal of National Defending Forces in Southwest China

基　　金：上海金山区科学技术创新基金(2012-3-06)

摘　　要：目的探讨青藤碱对人结肠癌SW620细胞增殖的影响,及其对TNF-α、IL-23及β-catenin表达水平的影响。方法体外培养的人结肠癌SW620细胞,经过不同浓度的青藤碱处理后,采用MTT法观察细胞增殖改变,应用ELISA法测定细胞培养液中TNF-α、IL-23的含量,Western blotting方法检测人结肠癌细胞中β-catenin的表达水平。结果与空白对照组相比,青藤碱显著抑制人结肠癌SW620细胞增殖,且呈现浓度与时间依赖性,不同浓度组别间差异有统计学意义(P<0.05);与空白对照组相比,青藤碱作用人结肠癌SW620细胞后,细胞上清中TNF-α、IL-23表达水平显著下调(P<0.01),且SW620细胞β-catenin表达水平也明显下降(P<0.05)。结论青藤碱抑制人结肠癌SW620细胞增殖,其机制可能与TNF-α、IL-23及β-catenin表达下调有关。Objective To explore the impacts of sinomenine on the proliferation of human colon cancer SW620 cells and on the expression levels of TNF-α, IL-23 and β-catenin. Methods For the human colon cancer SW620 cells cultured in vitro and treated with sinomenine of different concentrations, MTT method was applied to observe the change in the proliferation of cells, ELISA method was taken to detect the contents of TNF-α and IL-23 in cell culture fluid and Western blotting method was used to detect the expression level of β-catenin in human colon cancer cells. Results Compared with the blank control group, the sinomenine significantly inhibited the proliferation of human colon cancer SW620 cells, and there were concentration and time dependence and statistical difference among the groups of different concentrations（P 〈 0.05）; compared with the blank control group, after the action of sinomenine on human colon cancer SW620 cells, the expression levels of TNF-α and IL-23 in cell supernatant decreased significantly（P 〈 0.01） and the expression level of β-catenin in SW620 cells also decreased significantly（P 〈 0.05）. Conclusion Sinomenine can inhibit the prolifer ation of human colon cancer SW620 cells; its mechanism may be relevant to the down-regulated expression of TNF-α, IL-23 and β-catenin.

关 键 词：青藤碱 结肠癌 SW620细胞 TNF-α、IL-23 Β-CATENIN 细胞增殖 

分 类 号：R735.2[医药卫生—肿瘤]