黄芪总皂苷对心力衰竭大鼠钙转运的影响及机制探讨  

Discussion on the effect and its mechanism of Astragalosides on calcium transport in rats with chronic heart failure

作　　者：姬艳苏[1] 李亮 赵榕慧[1] 陈晓锐 朱铁梁[1] 

机构地区：[1]武警后勤学院附属医院,天津300162 [2]天津医科大学总医院,天津300052

出　　处：《现代中西医结合杂志》2018年第1期13-18,共6页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：天津市应用基础与前沿技术研究计划项目(14JCQNJC13600)

摘　　要：目的探讨黄芪总皂苷对心肌梗死后心力衰竭大鼠心肌细胞内钙离子浓度及心肌血管紧张素Ⅱ受体1(AT1)和磷脂酶C(PLC)的影响。方法取健康Wistar大鼠50只,从中随机选取8只作为假手术组,其余大鼠采用结扎左冠状动脉前降支方法复制心肌梗死后慢性心力衰竭大鼠模型。将造模成功的32只大鼠随机分为模型组、黄芪总皂苷10 mg/kg组、黄芪总皂苷20 mg/kg组和卡托普利50 mg/kg组,每组8只,各给药组分别给予相应剂量药物灌胃4周。分别于灌胃前和灌胃2周、4周取血检测血清心肌肌钙蛋白T(c Tn T)水平;灌胃4周后取各组大鼠心肌组织,采用免疫荧光技术观察心肌细胞内钙荧光强度以评价细胞内钙离子浓度,采用Western-Blot技术检测心肌组织中AT1和PLC蛋白表达水平,应用酶水解同位素底物法检测心肌组织中膜蛋白PLC的活性,采用PCR法检测心肌组织中AT1 mRNA表达水平。结果灌胃2周后,黄芪总皂苷20 mg/kg组和卡托普利50 mg/kg组大鼠血清心肌c Tn T水平均明显低于模型组(P均<0.05);灌胃4周后,各给药组大鼠血清心肌cTnT水平均明显低于模型组(P均<0.05),大鼠舒张期心肌细胞内Ca2+荧光强度明显弱于模型组。灌胃4周后,各给药组AT1mRNA表达水平及黄芪总皂苷20 mg/kg组、卡托普利50 mg/kg组AT1蛋白表达水平和PLC活性均明显低于模型组(P均<0.05),各组大鼠心肌组织中PLC蛋白表达水平比较差异均无统计学意义(P均>0.05)。结论黄芪总皂苷可降低心肌梗死后慢性心力衰竭大鼠血清c Tn T水平和心肌细胞内钙离子浓度,下调心肌组织中AT1蛋白和mRNA表达水平,降低心肌PLC的活性,其机制可能是通过调节AT1-PLC通路,减轻钙超载对细胞的损伤,从而改善心肌缺氧缺血。Objection It is to investigate the effects of Astragalosides （AS） on Ca2＋ concentration and angiotensin II type 1 receptor（ AT1 ） and phospholipase C （PLC） in the rats with myocardial ischemia with chronic heart failure. Methods Fifty healthy Wistar rats were selected, in which 8 rats was selected as sham operation group, the other rats were selected to establish the models of myocardial ischemia with chronic heart failure by ligaturing the left anterior descending branch of coronary artery and were randomly divided into model group, 10 mg/kg AS group, 20 mg/kg AS group and 50 mg/kg Cap- topril group after successful establishing, each group had 8 rats and were treated with respective medicine by lavage for 4 weeks. The concentration of cardiac troponin T （cTnT） in serum was detected by enzyme linked immunosorbent technology （ELISA） method before treatment, after treatment for 2 and 4 weeks; the Ca2＋ flourescence intensity in myocardial cells were detected after treatment for 4 weeks by immunofluorescence technique to evaluate Ca2 ＋ concentration in the cells, the expression levels of AT1 and PLC protein in myocardial tissue were detected by Western-Blot method, the activity of PLC and expression of AT1 mRNA in myocardial tissue were respectively detected by Enzyme hydrolysis isotope substrate method and RT-PCR technology. Results After treatment for 2 weeks, the levels of cTnT in serum in 20 mg/kg AS group and Cap- topril group were significantly lower than that in model group （ P 〈 0.05） ; after treatment for 4 weeks, the levels of cTnT inserum in every treatment group were significantly lower while Ca2＋ flourescence intensity in myocardial ceils in diastolic phase were significantly less than that in model group （P 〈 0.05）. After treatment for 4 weeks, the expression levels of AT1 mRNA in every treatment group, the expression levels of ATI protein and PLC activity in 20 mg/kg AS group, 50 nag/ kg Captopril group were significantly lower than that in model group （P 〈

关 键 词：黄芪总皂苷 慢性心力衰竭 钙离子浓度 血管紧张素Ⅱ受体1 磷脂酶C 

分 类 号：R-332[医药卫生]