唾液链球菌抑制变形链球菌生物膜作用的发酵条件优化  

Optimal cultivation conditions for Streptococcus salivarius BD3900 to express substances capable of inhibiting Streptococcus mutans biofilm formation

作　　者：于亚男 吴正钧 韩瑨[3,4] 

机构地区：[1]乳业生物技术国家重点实验室,上海200436 [2]上海乳业生物工程技术研究中心,上海200436 [3]光明乳业股份有限公司光明乳业研究院,上海200436 [4]上海海洋大学食品学院,上海201306

出　　处：《食品与机械》2017年第10期34-38,51,共6页Food and Machinery

基　　金：上海市闵行区重大产业技术攻关项目(编号:2015MH093);上海乳业生物工程技术研究中心(编号:16DZ2280600)

摘　　要：为提高唾液链球菌BD3900代谢产物对变形链球菌生物膜的抑制作用,以变形链球菌生物膜形成量的抑制率为指标,分别考察了培养时间、培养温度、碳源种类、蔗糖浓度、接种量5个因素对BD3900抑制变形链球菌生物膜的影响。结果表明,优化后的培养条件为:培养时间16h、培养温度34℃、蔗糖浓度1 g/100 mL。经过发酵条件优化后,BD3900代谢产物对变形链球菌生物膜的抑制作用相比优化前提高了10%,其作用机制可能是减少变形链球菌生物膜中不溶性胞外多糖的产生。In order to increase the metabolites of the strain BD3900 inhibiting the S. mutans biofilm formation, the inhibition rate of bio- film formation by Streptococcus mutans was used as indexes. The effects of the cultivation time and temperature, the carbon resources, the concentration of sucrose and the inoculum amount were investigated. The results showed that optimal cultivation conditions were composed of a cultivation period of 16 h, a cultivation temperature of 34℃, and a sucrose concentration of 1 g/100 mL. Under the optimized cultivation conditions, the inhibitory ability of the strain BD3900 on the formation of S. mutans biofilm was increased by 10%, compared with the results before optimization. The mechanism involved in the inhibiting of S. mutans biofilm formation by the strain BD3900 was probably decreasing the insoluble exopo lysaccharides synthetization.

关 键 词：唾液链球菌BD3900 变形链球菌 生物膜形成量 培养条件 不溶性胞外多糖 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]